Easy washing of lysed cell plugs for bacterial typing by pulsed-field gel electrophoresis using simple equipment

Koichi Murakami, Tamie Noda, Eriko Maeda, Nobuyuki Sera, Shuji Fujimoto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We designed and tested equipment to wash plugs following cell lysis in pulsed-field gel electrophoresis (PFGE). Our system can wash 30 plugs simultaneously in 1. h using 15. L of Tris-EDTA buffer, which makes plug washing for PFGE less labor-intensive.

Original languageEnglish
Pages (from-to)67-69
Number of pages3
JournalJournal of Microbiological Methods
Volume101
Issue number1
DOIs
Publication statusPublished - Jun 2014

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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