Easy washing of lysed cell plugs for bacterial typing by pulsed-field gel electrophoresis using simple equipment

Koichi Murakami; Tamie Noda; Eriko Maeda; Nobuyuki Sera; Shuji Fujimoto

doi:10.1016/j.mimet.2014.04.001

Easy washing of lysed cell plugs for bacterial typing by pulsed-field gel electrophoresis using simple equipment

Koichi Murakami, Tamie Noda, Eriko Maeda, Nobuyuki Sera, Shuji Fujimoto

Division of Medical Technology

Research output: Contribution to journal › Article

3 Citations (Scopus)

Abstract

We designed and tested equipment to wash plugs following cell lysis in pulsed-field gel electrophoresis (PFGE). Our system can wash 30 plugs simultaneously in 1. h using 15. L of Tris-EDTA buffer, which makes plug washing for PFGE less labor-intensive.

Original language	English
Pages (from-to)	67-69
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	101
Issue number	1
DOIs	https://doi.org/10.1016/j.mimet.2014.04.001
Publication status	Published - Jun 2014

All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/j.mimet.2014.04.001

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Murakami, K., Noda, T., Maeda, E., Sera, N., & Fujimoto, S. (2014). Easy washing of lysed cell plugs for bacterial typing by pulsed-field gel electrophoresis using simple equipment. Journal of Microbiological Methods, 101(1), 67-69. https://doi.org/10.1016/j.mimet.2014.04.001

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abstract = "We designed and tested equipment to wash plugs following cell lysis in pulsed-field gel electrophoresis (PFGE). Our system can wash 30 plugs simultaneously in 1. h using 15. L of Tris-EDTA buffer, which makes plug washing for PFGE less labor-intensive.",

author = "Koichi Murakami and Tamie Noda and Eriko Maeda and Nobuyuki Sera and Shuji Fujimoto",

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