Ecophysiology of Zetaproteobacteria associated with shallow hydrothermal iron-oxyhydroxide deposits in Nagahama Bay of Satsuma Iwo-Jima, Japan

Tatsuhiko Hoshino, Takashi Kuratomi, Yuki Morono, Tomoyuki Hori, Hisashi Oiwane, Shoichi Kiyokawa, Fumio Inagaki

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Abstract

Previous studies of microbial communities in deep-sea hydrothermal ferric deposits have demonstrated that members of Zetaproteobacteria play significant ecological roles in biogeochemical iron-cycling. However, the ecophysiological characteristics and interaction between other microbial members in the habitat still remain largely unknown. In this study, we investigated microbial communities in a core sample obtained from shallow hydrothermal iron-oxyhydroxide deposits at Nagahama Bay of Satsuma Iwo-Jima, Japan. Scanning electron microscopic observation showed numerous helical stalk structures, suggesting the occurrence of iron-oxidizing bacteria. Analysis of 16S rRNA gene sequences indicated the co-occurrence of iron-oxidizing Zetaproteobacteria and iron-reducing bacteria such as the genera Deferrisoma and Desulfobulbus with strong correlations on the sequence abundance. CARD-FISH indicated that the numbers of Zetaproteobacteria were not always consistent to the frequency of stalk structures. In the stalk-abundant layers with relatively small numbers of Zetaproteobacteria cells, accumulation of polyphosphate was observed inside Zetaproteobacteria cells, whereas no polyphosphate grains were observed in the topmost layers with fewer stalks and abundant Zetaproteobacteria cells. These results suggest that Zetaproteobacteria store intracellular polyphosphates during active iron oxidation that contributes to the mineralogical growth and biogeochemical iron cycling.

Original languageEnglish
Article number1554
JournalFrontiers in Microbiology
Volume6
Issue numberJAN
DOIs
Publication statusPublished - Jan 1 2016

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Japan
Iron
Polyphosphates
Bacteria
rRNA Genes
Oceans and Seas
Ecosystem
ferric hydroxide
Cell Count
Electrons
Growth

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

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Ecophysiology of Zetaproteobacteria associated with shallow hydrothermal iron-oxyhydroxide deposits in Nagahama Bay of Satsuma Iwo-Jima, Japan. / Hoshino, Tatsuhiko; Kuratomi, Takashi; Morono, Yuki; Hori, Tomoyuki; Oiwane, Hisashi; Kiyokawa, Shoichi; Inagaki, Fumio.

In: Frontiers in Microbiology, Vol. 6, No. JAN, 1554, 01.01.2016.

Research output: Contribution to journalArticle

Hoshino, Tatsuhiko ; Kuratomi, Takashi ; Morono, Yuki ; Hori, Tomoyuki ; Oiwane, Hisashi ; Kiyokawa, Shoichi ; Inagaki, Fumio. / Ecophysiology of Zetaproteobacteria associated with shallow hydrothermal iron-oxyhydroxide deposits in Nagahama Bay of Satsuma Iwo-Jima, Japan. In: Frontiers in Microbiology. 2016 ; Vol. 6, No. JAN.
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