Ecto-ATP diphosphohydrolase (apyrase) in ovarian follicle cells of starfish Asterina pectinifera

Masatoshi Mita, Michiyasu Yoshikuni, Yoshitaka Nagahama

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A Mg2+-dependent ecto-ATP diphosphohydrolase (ATPDase, EC 3.6.1.5) was present in starfish ovarian follicle cells. The enzyme which had an optimum pH between 6.0 and 7.5 could hydrolyze triphosphonucleosides (ATP, dATP, GTP, CTP, UTP and ITP) and diphosphonucleosides (ADP, dADP, GDP, CDP, UDP and IDP), but not monophosphonucleosides. The K(m) values for ATP and ADP were 0.17 and 0.20 mM, respectively. The ATPDase activity was insensitive to specific ATPase inhibitors, such as ouabain, vanadate and oligomycin, an adenylate kinase inhibitor, P1, P5-di-(adenosine-5')pentaphosphate, and a phosphodiesterase inhibitor, 1-isobutyl-3-methylxanthine. The activity was mostly distributed in the membrane fraction of follicle cells. It is also interesting that hydrolysis of ATP and ADP occurred upon incubation of intact cells in seawater. Among various kinds of detergents, digitonin was capable of solubilizing the enzyme from the membrane fraction. Using digitonin extract, a high-performance liquid chromatography with Superose 6 column showed that the molecular weight of ecto-ATPDase in starfish follicle cells was ~60 000. Copyright (C) 1998 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)577-583
Number of pages7
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume119
Issue number3
DOIs
Publication statusPublished - Jan 1 1998

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Asterina
Starfish
Ovarian Follicle
Adenosine Diphosphate
Digitonin
Adenosine Triphosphate
Inosine Diphosphate
Cytidine Diphosphate
Inosine Triphosphate
Membranes
Oligomycins
Cytidine Triphosphate
1-Methyl-3-isobutylxanthine
Adenylate Kinase
Uridine Triphosphate
Uridine Diphosphate
Vanadates
Phosphodiesterase Inhibitors
High performance liquid chromatography
Ouabain

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology

Cite this

Ecto-ATP diphosphohydrolase (apyrase) in ovarian follicle cells of starfish Asterina pectinifera. / Mita, Masatoshi; Yoshikuni, Michiyasu; Nagahama, Yoshitaka.

In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 119, No. 3, 01.01.1998, p. 577-583.

Research output: Contribution to journalArticle

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