Ectopic expression of Hmgn2 antagonizes mouse erythroid differentiation in vitro

Kasem Kulkeaw, Tomoko Inoue, Chiyo Mizuochi, Yuka Horio, Yasushi Ishihama, Daisuke Sugiyama

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    Hmgn2 (high mobility group nucleosomal 2), a ubiquitous nucleosome-binding protein that unfolds chromatin fibres and enhances DNA replication, reportedly regulates differentiation of epithelial and mesenchymal cells. To investigate how Hmgn2 regulates HC (haemopoietic cell) differentiation, we quantified Hmgn2 expression in HCs of mouse FL (fetal liver) during erythroid differentiation. Hmgn2 expression levels were >10-fold higher in immature erythroid progenitors than in mature erythroid cells, suggesting that Hmgn2 antagonizes erythroid differentiation. To address this issue, Hmgn2 were transfected into both Friend erythroleukaemia cells and FL HCs. There was a 3.3-fold decrease in relatively mature c-Kit +/CD71 + erythroid cells, a 2.9-fold increase in immature c-Kit +/CD712 erythroid cells in transfected Friend cells, a 1.1-fold decrease in relatively mature CD71 +/Ter119 + erythroid cells, and a 1.7-fold increase in relatively immature c-Kit +/CD71 + erythroid cells in FL HCs accompanied by down-regulation of genes encoding the erythroid transcription factors, Gata1 and Klf1. Two days after Hmgn2 transfection of Friend erythroleukaemia cells, the number of S-phase cells increased, whereas the number of cells in G 1 decreased, while that of mitotic cells remained unchanged. We conclude that ectopic expression of Hmgn2 antagonizes mouse erythroid differentiation in vitro, which may be due to enhancement of DNA replication and/or blocking entry of mitosis at S-phase.

    Original languageEnglish
    Pages (from-to)195-202
    Number of pages8
    JournalCell Biology International
    Volume36
    Issue number2
    DOIs
    Publication statusPublished - Feb 1 2012

    All Science Journal Classification (ASJC) codes

    • Cell Biology

    Fingerprint Dive into the research topics of 'Ectopic expression of Hmgn2 antagonizes mouse erythroid differentiation in vitro'. Together they form a unique fingerprint.

    Cite this