We previously developed a hydrolase-based fluorescence amplification method for antigen-specific cell labelling, in which fluorescent substrates stained cells by a non-covalent hydrophobic interaction. To improve the substrates retention in cells, we examined the effect of a chloroacetyl group modification on the substrate retention. We found that the chloroacetyl group suppressed the dissociation of the substrate after forming a covalent bond with intracellular proteins. However’ the slow reaction speed of the chloroacetyl group allowed dissociation for cells in the early stage of the staining reaction.
All Science Journal Classification (ASJC) codes
- Analytical Chemistry