Effect of a Chloroacetyl Modification on the Suppression of Dissociation of a Fluorescent Molecule from Cells for Antigen-Specific Cell Staining

Ryosuke Kaneko, Masumi Kawamura, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

    Research output: Contribution to journalArticlepeer-review

    Abstract

    We previously developed a hydrolase-based fluorescence amplification method for antigen-specific cell labelling, in which fluorescent substrates stained cells by a non-covalent hydrophobic interaction. To improve the substrates retention in cells, we examined the effect of a chloroacetyl group modification on the substrate retention. We found that the chloroacetyl group suppressed the dissociation of the substrate after forming a covalent bond with intracellular proteins. However’ the slow reaction speed of the chloroacetyl group allowed dissociation for cells in the early stage of the staining reaction.

    Original languageEnglish
    Pages (from-to)529-532
    Number of pages4
    Journalanalytical sciences
    Volume37
    Issue number3
    DOIs
    Publication statusPublished - 2021

    All Science Journal Classification (ASJC) codes

    • Analytical Chemistry

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