Effect of an Antibacterial Factor Derived from Bacillus subtilis FHC 402 on the Growth of Bacteria and Mouse Myeloma MPC-11 Cells

Takahisa Miyamoto, Koji Yamada, Shoji Hatano

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The antibacterial activities of Bacillus subtilis FHC 402-derived antibacterial factor (BAF) and hexametaphosphate (HP) and the combined effects of BAF and HP were examined on the growth of 15 strains of bacteria causing food poisoning and spoilage. BAF was more effective on gram-negative bacteria than gram-positive ones. BAF inhibited the cell growth of L. heterohiochii, C. jejuni, V. parahaemolyticus, V. anguillarum and S. typhimurium in the concentration range from 100 to 800 unit/ml. The cell growth of bacteria resistant to BAF such as B. subtilis, B. cereus, L. lactis, E. coli and P. fragi was completely inhibited by the combined use of BAF and 0.1% HP. BAF prolonged the lag phase of the growth of E. coli treated with HP, though HP did not affect the growth of cells treated with BAF. These results suggest that the damage induced by HP facilitates the antibacterial action of BAF on E. coli. We also investigated the effects of BAF and/or HP on the growth and viability of mouse myeloma MPC-11 cells as preliminary tests on the toxicity of these substances. BAF inhibited the growth of MPC-11 cells and decreased the cell viability, but these effects were weaker than those of HP, which has relatively low toxicity in mammalia.

Original languageEnglish
Number of pages1
JournalFood Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
Volume28
Issue number5
DOIs
Publication statusPublished - Jan 1 1987

Fingerprint

myeloma
Bacillus subtilis
cell growth
Escherichia coli
Bacteria
bacteria
mice
Growth
toxicity
Cereus
food spoilage
Mammalia
cells
foodborne illness
Gram-negative bacteria
cell viability
viability
Toxicity Tests
Foodborne Diseases
hexametaphosphate

All Science Journal Classification (ASJC) codes

  • Food Science
  • Public Health, Environmental and Occupational Health

Cite this

@article{352d62bb116646c28d8575d6aaa45897,
title = "Effect of an Antibacterial Factor Derived from Bacillus subtilis FHC 402 on the Growth of Bacteria and Mouse Myeloma MPC-11 Cells",
abstract = "The antibacterial activities of Bacillus subtilis FHC 402-derived antibacterial factor (BAF) and hexametaphosphate (HP) and the combined effects of BAF and HP were examined on the growth of 15 strains of bacteria causing food poisoning and spoilage. BAF was more effective on gram-negative bacteria than gram-positive ones. BAF inhibited the cell growth of L. heterohiochii, C. jejuni, V. parahaemolyticus, V. anguillarum and S. typhimurium in the concentration range from 100 to 800 unit/ml. The cell growth of bacteria resistant to BAF such as B. subtilis, B. cereus, L. lactis, E. coli and P. fragi was completely inhibited by the combined use of BAF and 0.1{\%} HP. BAF prolonged the lag phase of the growth of E. coli treated with HP, though HP did not affect the growth of cells treated with BAF. These results suggest that the damage induced by HP facilitates the antibacterial action of BAF on E. coli. We also investigated the effects of BAF and/or HP on the growth and viability of mouse myeloma MPC-11 cells as preliminary tests on the toxicity of these substances. BAF inhibited the growth of MPC-11 cells and decreased the cell viability, but these effects were weaker than those of HP, which has relatively low toxicity in mammalia.",
author = "Takahisa Miyamoto and Koji Yamada and Shoji Hatano",
year = "1987",
month = "1",
day = "1",
doi = "10.3358/shokueishi.28.364",
language = "English",
volume = "28",
journal = "Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan",
issn = "0015-6426",
publisher = "Food Hygienic Society of Japan",
number = "5",

}

TY - JOUR

T1 - Effect of an Antibacterial Factor Derived from Bacillus subtilis FHC 402 on the Growth of Bacteria and Mouse Myeloma MPC-11 Cells

AU - Miyamoto, Takahisa

AU - Yamada, Koji

AU - Hatano, Shoji

PY - 1987/1/1

Y1 - 1987/1/1

N2 - The antibacterial activities of Bacillus subtilis FHC 402-derived antibacterial factor (BAF) and hexametaphosphate (HP) and the combined effects of BAF and HP were examined on the growth of 15 strains of bacteria causing food poisoning and spoilage. BAF was more effective on gram-negative bacteria than gram-positive ones. BAF inhibited the cell growth of L. heterohiochii, C. jejuni, V. parahaemolyticus, V. anguillarum and S. typhimurium in the concentration range from 100 to 800 unit/ml. The cell growth of bacteria resistant to BAF such as B. subtilis, B. cereus, L. lactis, E. coli and P. fragi was completely inhibited by the combined use of BAF and 0.1% HP. BAF prolonged the lag phase of the growth of E. coli treated with HP, though HP did not affect the growth of cells treated with BAF. These results suggest that the damage induced by HP facilitates the antibacterial action of BAF on E. coli. We also investigated the effects of BAF and/or HP on the growth and viability of mouse myeloma MPC-11 cells as preliminary tests on the toxicity of these substances. BAF inhibited the growth of MPC-11 cells and decreased the cell viability, but these effects were weaker than those of HP, which has relatively low toxicity in mammalia.

AB - The antibacterial activities of Bacillus subtilis FHC 402-derived antibacterial factor (BAF) and hexametaphosphate (HP) and the combined effects of BAF and HP were examined on the growth of 15 strains of bacteria causing food poisoning and spoilage. BAF was more effective on gram-negative bacteria than gram-positive ones. BAF inhibited the cell growth of L. heterohiochii, C. jejuni, V. parahaemolyticus, V. anguillarum and S. typhimurium in the concentration range from 100 to 800 unit/ml. The cell growth of bacteria resistant to BAF such as B. subtilis, B. cereus, L. lactis, E. coli and P. fragi was completely inhibited by the combined use of BAF and 0.1% HP. BAF prolonged the lag phase of the growth of E. coli treated with HP, though HP did not affect the growth of cells treated with BAF. These results suggest that the damage induced by HP facilitates the antibacterial action of BAF on E. coli. We also investigated the effects of BAF and/or HP on the growth and viability of mouse myeloma MPC-11 cells as preliminary tests on the toxicity of these substances. BAF inhibited the growth of MPC-11 cells and decreased the cell viability, but these effects were weaker than those of HP, which has relatively low toxicity in mammalia.

UR - http://www.scopus.com/inward/record.url?scp=85007947266&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85007947266&partnerID=8YFLogxK

U2 - 10.3358/shokueishi.28.364

DO - 10.3358/shokueishi.28.364

M3 - Article

AN - SCOPUS:85007947266

VL - 28

JO - Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan

JF - Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan

SN - 0015-6426

IS - 5

ER -