Effect of antibiotics on extracellular protein level in Pseudomonas aeruginosa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Pseudomonas aeruginosa PAO1 organisms harbouring different plasmids were cultured in broths containing appropriate antibiotic(s). Extracellular proteins were more abundant in the presence of tetracycline or kanamycin than in the presence of other antibiotics. Zymography revealed that alkaline protease (AprA) production was interfered by these antibiotics. Extracellular proteins were not observed at the same level when AprA-deficient EG03 strains were cultured in the presence of different antibiotics. The extracellular protein levels were dependent on the antibiotics and plasmid derivative groups. Levels of extracellular protein were not significantly different between PAO1 (pBBR1MCS-5) and EG03 (pAprcomp-MCS5), and profiles of the extracellular proteome were comparable. In contrast, the level of EG03 (pBBR1MCS-MCS5) extracellular protein was higher than those observed in the other two strains. These results suggested that although AprA partially contributes to the alteration of extracellular protein level, the effect is limited.

Original languageEnglish
Pages (from-to)44-50
Number of pages7
JournalPlasmid
Volume84-85
DOIs
Publication statusPublished - Mar 1 2016

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Pseudomonas aeruginosa
Anti-Bacterial Agents
Proteins
Plasmids
Kanamycin
Proteome
Tetracycline
alkaline protease

All Science Journal Classification (ASJC) codes

  • Molecular Biology

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Effect of antibiotics on extracellular protein level in Pseudomonas aeruginosa. / Takahashi, Eigo; Lee, Jae Man; Mon, Hiroaki; Chieda, Yuuka; Yasunaga-Aoki, Chisa; Kusakabe, Takahiro; Iiyama, Kazuhiro.

In: Plasmid, Vol. 84-85, 01.03.2016, p. 44-50.

Research output: Contribution to journalArticle

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AU - Lee, Jae Man

AU - Mon, Hiroaki

AU - Chieda, Yuuka

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AU - Kusakabe, Takahiro

AU - Iiyama, Kazuhiro

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