Effect of Arg145Gly mutation in human cardiac troponin I on the ATPase activity of cardiac myofibrils

Fumi Takahashi-Yanaga, Sachio Morimoto, Iwao Ohtsuki

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Abstract

In order to determine the functional consequences of the Arg145Gly mutation in troponin I found in familial hypertrophic cardiomyopathy, human cardiac troponin I and its mutant were expressed in Escherichia coli and purified, and then their effects on the ATPase activity of porcine cardiac myofibrillar preparations from which both troponins C and I had been depleted were examined. Both the wild-type and mutant troponin is suppressed the ATPase activity of the troponin C·I-depleted myofibrils, but the maximum inhibition caused by mutant troponin I was weaker than that by wild-type troponin I. In the Ca2+-activation profile of the myofibrillar ATPase activity after reconstitution with both troponins I and C, the Ca2+- sensitivity with mutant troponin I was higher than that with wild-type troponin I, whereas the maximum level of the ATPase activity with mutant troponin I was lower than that with wild-type troponin I. These findings strongly suggest that the Arg145Gly mutation in human cardiac troponin I modulates the Ca2+-regulation of contraction by impairing the interaction of troponin I with both actin-tropomyosin and troponin C.

Original languageEnglish
Pages (from-to)355-357
Number of pages3
JournalJournal of biochemistry
Volume127
Issue number3
DOIs
Publication statusPublished - Jan 1 2000

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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