Effect of Azo Dyes on Proliferation in Mouse Embryo Culture Cells

Yoshiko Okawa, Makoto Yoshimoto, Takahisa Miyamoto, Shoji Hatano

Research output: Contribution to journalArticle

Abstract

Amaranth (Food Red No. 2) and Ponceau 3R stimulate in vitro RNA synthesis by causing the dissociation of chromatin in isolated rat-liver nuclei. The effects of these azo dyes and Sunset Yellow FCF (Food Yellow No. 5) on proliferation of mouse embryo culture cells, C3H10 T1/2, were studied. 1) When the cells were pretreated for 1 hr with S9-untreated azo dyes at concentrations of 0.3. 1.0, and 5 mM, the cell growth was slightly reduced by 5 mM Ponceau 3R. All the S9 -untreated azo dyes inhibited cell growth when added to the medium during cultivation. 2) The 1-hr pretreatment of the cells with S9-treated amaranth and Ponceau 3R at a concentration of 0.3 mM temporarily accelerated the cell growth in the early stage of cell proliferation. However, this was not observed when these S9-treated azo dyes were added to the medium during cultivation.

Original languageEnglish
Number of pages1
JournalFood Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
Volume30
Issue number6
DOIs
Publication statusPublished - Jan 1 1989

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Azo Compounds
azo dyes
embryo culture
Embryonic Structures
Cell Culture Techniques
cell growth
mice
cells
Growth
Food
chromatin
cell proliferation
pretreatment
Chromatin
RNA
liver
synthesis
Cell Proliferation
rats
Liver

All Science Journal Classification (ASJC) codes

  • Food Science
  • Public Health, Environmental and Occupational Health

Cite this

Effect of Azo Dyes on Proliferation in Mouse Embryo Culture Cells. / Okawa, Yoshiko; Yoshimoto, Makoto; Miyamoto, Takahisa; Hatano, Shoji.

In: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi), Vol. 30, No. 6, 01.01.1989.

Research output: Contribution to journalArticle

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