TY - JOUR
T1 - Effect of Branching Degree of Dendritic Polyglycerols on Plasma Protein Adsorption
T2 - Relationship between Hydration States and Surface Morphology
AU - Yamazaki, Moe
AU - Sugimoto, Yosuke
AU - Murakami, Daiki
AU - Tanaka, Masaru
AU - Ooya, Tooru
N1 - Funding Information:
This work was performed under the Research Program of “Biocompatibility evaluation of nanosized soft materials with excellent reliability using water as an index” and “Dynamic Alliance for Open Innovation Bridging Human, Environment, and Materials” in “Network Joint Research Center for Materials and Devices, Japan”. This work was partially supported by a Grant-in-Aid for Scientific Research B (JSPS KAKENHI grant number 22300165), a Grant-in-Aid for Scientific Research on Innovative Areas “New Polymeric Materials Based on Element-Blocks (no. 2401)” (JSPS KAKENHI grant number JP15H00748), and Grants-in-Aid for Scientific Research (JSPS KAKENHI grant number JP19H05720).
Publisher Copyright:
©
PY - 2021/7/20
Y1 - 2021/7/20
N2 - This study focuses on dendritic glycerols and investigates the construction of biocompatible surfaces by understanding how differences in the branching of these molecules change the interactions with the biological components. The two molecules, polyglycerol dendrimer (PGD), which has a completely branched structure, and hyperbranched polyglycerol (HPG), which has an incompletely branched structure, are compared and the differences in branching are evaluated. It is shown that PGD has a little bit more intermediate water than HPG, which reflects the differences in the branching. The effect of surface state on the adsorption of the plasma proteins, human serum albumin (HSA), fibrinogen (Fib), and fibronectin (FN), is discussed by modifying a glass surface using these molecules with different hydration states. The adsorption of HSA decreases to several percent for HPG and 10% for PGD compared to unmodified substrate. Although the adsorption of Fib decreases to 5% for HPG, an increase to 150% is observed for PGD. Since this specific Fib adsorption observed only onto PGD is suppressed in the cases of a mixed solution of HSA and Fib or sequentially using HSA solution and then Fib solution, it is thought that the Vroman effect is suppressed on the PGD-modified surface. Furthermore, when AFM measurements are performed in PBS to understand the surface roughness, PGD is found to be more highly non-uniform. Because of this, the nanometer scale roughness that is significantly observed only on the PGD-modified surface is thought to have an effect on the characteristic adsorption properties of Fib. Thus, although both PGD and HPG with different branching have intermediate water, the proportion differs between PGD and HPG. Therefore, it is found that differences occur in the plasma protein adsorption mechanisms depending on the coordinates and density of hydroxyl groups within the molecules.
AB - This study focuses on dendritic glycerols and investigates the construction of biocompatible surfaces by understanding how differences in the branching of these molecules change the interactions with the biological components. The two molecules, polyglycerol dendrimer (PGD), which has a completely branched structure, and hyperbranched polyglycerol (HPG), which has an incompletely branched structure, are compared and the differences in branching are evaluated. It is shown that PGD has a little bit more intermediate water than HPG, which reflects the differences in the branching. The effect of surface state on the adsorption of the plasma proteins, human serum albumin (HSA), fibrinogen (Fib), and fibronectin (FN), is discussed by modifying a glass surface using these molecules with different hydration states. The adsorption of HSA decreases to several percent for HPG and 10% for PGD compared to unmodified substrate. Although the adsorption of Fib decreases to 5% for HPG, an increase to 150% is observed for PGD. Since this specific Fib adsorption observed only onto PGD is suppressed in the cases of a mixed solution of HSA and Fib or sequentially using HSA solution and then Fib solution, it is thought that the Vroman effect is suppressed on the PGD-modified surface. Furthermore, when AFM measurements are performed in PBS to understand the surface roughness, PGD is found to be more highly non-uniform. Because of this, the nanometer scale roughness that is significantly observed only on the PGD-modified surface is thought to have an effect on the characteristic adsorption properties of Fib. Thus, although both PGD and HPG with different branching have intermediate water, the proportion differs between PGD and HPG. Therefore, it is found that differences occur in the plasma protein adsorption mechanisms depending on the coordinates and density of hydroxyl groups within the molecules.
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U2 - 10.1021/acs.langmuir.1c01003
DO - 10.1021/acs.langmuir.1c01003
M3 - Article
C2 - 34223767
AN - SCOPUS:85110997984
VL - 37
SP - 8534
EP - 8543
JO - Langmuir
JF - Langmuir
SN - 0743-7463
IS - 28
ER -