Effect of cell permeable peptide of c-Jun NH2-terminal kinase inhibitor on the attenuation of renal ischemia-reperfusion injury in pigs

A. Doi, H. Kitada, M. Ota, S. Kawanami, K. Kurihara, Y. Miura, T. Nishiki, Y. Okabe, S. Inoue, M. Tanaka

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The outcomes of organ transplantation have improved due to better immunosuppressive drugs, surgical techniques, and management of complications. However, ischemia-reperfusion injury remains a challenge affecting graft survival. In this study, we employed injection of a protein transduction domain (PTD) to inhibit the c-Jun NH2-terminal kinase (JNK) pathway thereby attenuating ischemia-reperfusion injury in a porcine model. The PTD-JNK inhibitor (JNKI) was administered into the renal artery, allowing it to be taken into various elements including vascular endothelial cells by endocytosis via the PTD. Serum creatinine and blood urea nitrogen concentrations were lower among PTD-JNKI than controls. In addition, renal tissue blood flow was maintained in the PTD-JNKI group, resulting in less tissue injury and fewer apoptotic cells. These results suggested that the PTD technique improved renal transplantation outcomes.

Original languageEnglish
Pages (from-to)2469-2475
Number of pages7
JournalTransplantation Proceedings
Volume45
Issue number6
DOIs
Publication statusPublished - Jul 1 2013

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JNK Mitogen-Activated Protein Kinases
Reperfusion Injury
Swine
Kidney
Peptides
Renal Circulation
Blood Urea Nitrogen
Organ Transplantation
Graft Survival
Renal Artery
Immunosuppressive Agents
Endocytosis
Kidney Transplantation
Protein Domains
Creatinine
Endothelial Cells
Injections
Wounds and Injuries
Serum
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Surgery
  • Transplantation

Cite this

Effect of cell permeable peptide of c-Jun NH2-terminal kinase inhibitor on the attenuation of renal ischemia-reperfusion injury in pigs. / Doi, A.; Kitada, H.; Ota, M.; Kawanami, S.; Kurihara, K.; Miura, Y.; Nishiki, T.; Okabe, Y.; Inoue, S.; Tanaka, M.

In: Transplantation Proceedings, Vol. 45, No. 6, 01.07.2013, p. 2469-2475.

Research output: Contribution to journalArticle

Doi, A. ; Kitada, H. ; Ota, M. ; Kawanami, S. ; Kurihara, K. ; Miura, Y. ; Nishiki, T. ; Okabe, Y. ; Inoue, S. ; Tanaka, M. / Effect of cell permeable peptide of c-Jun NH2-terminal kinase inhibitor on the attenuation of renal ischemia-reperfusion injury in pigs. In: Transplantation Proceedings. 2013 ; Vol. 45, No. 6. pp. 2469-2475.
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