Abstract
We evaluated the effect of conjugated linoleic acid (CLA) isomers on the growth factor-induced proliferation of human breast cancer MCF-7 cells. When MCF-7 cells were cultured in RPMI 1640 medium supplemented with 1% fetal bovine serum (FBS), CLA inhibited the proliferation and notably cis9, trans11 (c9,t11)-CLA showed the strongest effect. However, cells barely grew when cultured with 1% charcoal-treated FBS (cFBS). Proliferation was promoted in cFBS cultured cells by the addition of 17β-estradiol (E2), insulin, and epidermal growth factor (EGF). Trans10, cis12 (T10,c12)-CLA inhibited cell proliferation induced by E2 and insulin, but not by EGF. T10,c12-CLA also exhibited cell-killing activity when cells were induced with insulin. On the other hand, c9,t11-CLA was shown to have no effect on MCF-7 cell proliferation induced by and of these three growth factors. In conclusion, although both c9, t11 and t10, c12-CLA can inhibit the proliferation of MCF-7 cells, our results suggested that they have separate mechanisms and different targets of actions.
| Original language | English |
|---|---|
| Pages (from-to) | 81-87 |
| Number of pages | 7 |
| Journal | Cancer Letters |
| Volume | 202 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Dec 8 2003 |
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All Science Journal Classification (ASJC) codes
- Oncology
- Cancer Research
Cite this
Effect of conjugated linoleic acid isomers on growth factor-induced proliferation of human breast cancer cells. / Chujo, Hitomi; Yamasaki, Masao; Nou, Shinsuke; Koyanagi, Nami; Tachibana, Hirofumi; Yamada, Koji.
In: Cancer Letters, Vol. 202, No. 1, 08.12.2003, p. 81-87.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of conjugated linoleic acid isomers on growth factor-induced proliferation of human breast cancer cells
AU - Chujo, Hitomi
AU - Yamasaki, Masao
AU - Nou, Shinsuke
AU - Koyanagi, Nami
AU - Tachibana, Hirofumi
AU - Yamada, Koji
PY - 2003/12/8
Y1 - 2003/12/8
N2 - We evaluated the effect of conjugated linoleic acid (CLA) isomers on the growth factor-induced proliferation of human breast cancer MCF-7 cells. When MCF-7 cells were cultured in RPMI 1640 medium supplemented with 1% fetal bovine serum (FBS), CLA inhibited the proliferation and notably cis9, trans11 (c9,t11)-CLA showed the strongest effect. However, cells barely grew when cultured with 1% charcoal-treated FBS (cFBS). Proliferation was promoted in cFBS cultured cells by the addition of 17β-estradiol (E2), insulin, and epidermal growth factor (EGF). Trans10, cis12 (T10,c12)-CLA inhibited cell proliferation induced by E2 and insulin, but not by EGF. T10,c12-CLA also exhibited cell-killing activity when cells were induced with insulin. On the other hand, c9,t11-CLA was shown to have no effect on MCF-7 cell proliferation induced by and of these three growth factors. In conclusion, although both c9, t11 and t10, c12-CLA can inhibit the proliferation of MCF-7 cells, our results suggested that they have separate mechanisms and different targets of actions.
AB - We evaluated the effect of conjugated linoleic acid (CLA) isomers on the growth factor-induced proliferation of human breast cancer MCF-7 cells. When MCF-7 cells were cultured in RPMI 1640 medium supplemented with 1% fetal bovine serum (FBS), CLA inhibited the proliferation and notably cis9, trans11 (c9,t11)-CLA showed the strongest effect. However, cells barely grew when cultured with 1% charcoal-treated FBS (cFBS). Proliferation was promoted in cFBS cultured cells by the addition of 17β-estradiol (E2), insulin, and epidermal growth factor (EGF). Trans10, cis12 (T10,c12)-CLA inhibited cell proliferation induced by E2 and insulin, but not by EGF. T10,c12-CLA also exhibited cell-killing activity when cells were induced with insulin. On the other hand, c9,t11-CLA was shown to have no effect on MCF-7 cell proliferation induced by and of these three growth factors. In conclusion, although both c9, t11 and t10, c12-CLA can inhibit the proliferation of MCF-7 cells, our results suggested that they have separate mechanisms and different targets of actions.
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UR - http://www.scopus.com/inward/citedby.url?scp=0344395637&partnerID=8YFLogxK
U2 - 10.1016/S0304-3835(03)00478-6
DO - 10.1016/S0304-3835(03)00478-6
M3 - Article
C2 - 14643029
AN - SCOPUS:0344395637
VL - 202
SP - 81
EP - 87
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 1
ER -