Effect of conjugated linoleic acid isomers on growth factor-induced proliferation of human breast cancer cells

Hitomi Chujo, Masao Yamasaki, Shinsuke Nou, Nami Koyanagi, Hirofumi Tachibana, Koji Yamada

Research output: Contribution to journalArticle

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Abstract

We evaluated the effect of conjugated linoleic acid (CLA) isomers on the growth factor-induced proliferation of human breast cancer MCF-7 cells. When MCF-7 cells were cultured in RPMI 1640 medium supplemented with 1% fetal bovine serum (FBS), CLA inhibited the proliferation and notably cis9, trans11 (c9,t11)-CLA showed the strongest effect. However, cells barely grew when cultured with 1% charcoal-treated FBS (cFBS). Proliferation was promoted in cFBS cultured cells by the addition of 17β-estradiol (E2), insulin, and epidermal growth factor (EGF). Trans10, cis12 (T10,c12)-CLA inhibited cell proliferation induced by E2 and insulin, but not by EGF. T10,c12-CLA also exhibited cell-killing activity when cells were induced with insulin. On the other hand, c9,t11-CLA was shown to have no effect on MCF-7 cell proliferation induced by and of these three growth factors. In conclusion, although both c9, t11 and t10, c12-CLA can inhibit the proliferation of MCF-7 cells, our results suggested that they have separate mechanisms and different targets of actions.

Original languageEnglish
Pages (from-to)81-87
Number of pages7
JournalCancer Letters
Volume202
Issue number1
DOIs
Publication statusPublished - Dec 8 2003

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Conjugated Linoleic Acids
Intercellular Signaling Peptides and Proteins
Breast Neoplasms
MCF-7 Cells
Charcoal
Insulin
Epidermal Growth Factor
Cell Proliferation
Serum
Estradiol
Cultured Cells

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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Effect of conjugated linoleic acid isomers on growth factor-induced proliferation of human breast cancer cells. / Chujo, Hitomi; Yamasaki, Masao; Nou, Shinsuke; Koyanagi, Nami; Tachibana, Hirofumi; Yamada, Koji.

In: Cancer Letters, Vol. 202, No. 1, 08.12.2003, p. 81-87.

Research output: Contribution to journalArticle

Chujo, Hitomi ; Yamasaki, Masao ; Nou, Shinsuke ; Koyanagi, Nami ; Tachibana, Hirofumi ; Yamada, Koji. / Effect of conjugated linoleic acid isomers on growth factor-induced proliferation of human breast cancer cells. In: Cancer Letters. 2003 ; Vol. 202, No. 1. pp. 81-87.
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