Effect of culture conditions on growth and lipase production by a newly isolated strain, Geotrichum-like R59 (Basidiomycetes)

Gryzyna Ginalska, Hee Yeon Cho, Nam Seok Cho, Renata Bancerz, Teresa Korniłowicz, Andrzej Leonowicz, Soo Jeong Shin, Shoji Ohga

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13 Citations (Scopus)

Abstract

Growth and production of lipase by a new isolant Geotrichum-like R59 were studied. Production of extracellular lipase was substantially enhanced when the initial pH of the culture medium, types of carbon and nitrogen sources, those of inducers of lipase biosynthesis, temperature and time of growth were optimized. Sucrose and triolein were the most effective compounds for lipase production. Maximum lipase production was obtained when urea was used as nitrogen source (146 U/ml). Temperature of 30°C, the initial pH of 6.0 and incubation time of 48 h were found as optimum conditions for cell growth and production of lipase by Geotrichum-hke R59 strain. The enzyme presented a good thermostability and still exhibited very high activity after 1 h incubation at 60°C.

Original languageEnglish
Pages (from-to)29-34
Number of pages6
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume52
Issue number1
Publication statusPublished - Feb 2007

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

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