Effect of deglycosylation of N-linked sugar chains on glucose oxidase from Aspergillus niger.

Kaoru Takegawa, K. Fujiwara, S. Iwahara, K. Yamamoto, T. Tochikura

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Endo-beta-N-acetylglucosaminidase from Flavobacterium sp. released about 30% of the N-linked sugar chains from the glucose oxidase of Aspergillus niger. To elucidate the role of the carbohydrate moiety, the enzymatic properties of native and carbohydrate-depleted glucose oxidases were compared. It was found that their catalytic activities and thermal and pH stabilities were identical. However, the carbohydrate-depleted glucose oxidase was more rapidly precipitated by the addition of trichloroacetic acid and ammonium sulfate than the native enzyme. These results show that the N-linked sugar chains of the glucose oxidase contributed to the high solubility of the enzyme in water.

Original languageEnglish
Pages (from-to)460-464
Number of pages5
JournalBiochemistry and cell biology = Biochimie et biologie cellulaire
Volume67
Issue number8
DOIs
Publication statusPublished - Jan 1 1989

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Glucose Oxidase
Aspergillus niger
Aspergillus
Sugars
Carbohydrates
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
Flavobacterium
Acetylglucosaminidase
Trichloroacetic Acid
Ammonium Sulfate
Enzymes
Solubility
Catalyst activity
Hot Temperature
Water

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Effect of deglycosylation of N-linked sugar chains on glucose oxidase from Aspergillus niger. / Takegawa, Kaoru; Fujiwara, K.; Iwahara, S.; Yamamoto, K.; Tochikura, T.

In: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 67, No. 8, 01.01.1989, p. 460-464.

Research output: Contribution to journalArticle

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