Effect of dehydroepiandrosterone on glucose uptake in cultured human fibroblasts

Naoki Nakashima, Masafumi Haji, Yoshiyuki Sakai, Yasuhiro Ono, Fumio Umeda, Hajime Nawata

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Dehydroepiandrosterone (DHEA) and its sulfate derivative (DHEA-S) reportedly have antidiabetic and antiobesity effects. The effect of DHEA on glucose uptake in cultured human fibroblasts was examined. Incubation of cells with supraphysiologic concentrations of DHEA (10-5 mol/L) for ≥10 hours enhanced 2-deoxyglucose (2-DG) uptake significantly (P < .05). Supraphysiologic concentrations of insulin (10-7 mol/L) increased the sensitivity of glucose uptake to DHEA. Conversely, the sensitivity of glucose uptake to insulin was increased by incubating cells with 10-6 mol/L DHEA. Both the abundance of transcripts encoding glucose transporter-1 (Glut-1) and the maximal velocity (Vmax) of 2-DG transport were increased in cultured fibroblasts incubated with DHEA. Cultured fibroblasts expressed a specific binding factor with low affinity for [3H]DHEA (maximal number of binding sites, 18,496 sites per cell; Kd, 298 nmol/L). Other androgen hormones exerted a less-marked effect on glucose uptake; DHEA-S had no effect. These results suggested that DHEA increases Glut-1 mRNA through binding to a specific factor in cultured human fibroblasts and thereby stimulates glucose uptake in these cells.

Original languageEnglish
Pages (from-to)543-548
Number of pages6
JournalMetabolism
Volume44
Issue number4
DOIs
Publication statusPublished - Apr 1995

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Fingerprint Dive into the research topics of 'Effect of dehydroepiandrosterone on glucose uptake in cultured human fibroblasts'. Together they form a unique fingerprint.

  • Cite this