TY - JOUR
T1 - Effect of dietary conjugated linoleic acid on the in vivo growth of rat hepatoma dRLh-84
AU - Yamasaki, Masao
AU - Ikeda, Atsushi
AU - Hirao, Akira
AU - Tanaka, Yoko
AU - Miyazaki, Yoshiyuki
AU - Rikimaru, Tatsuya
AU - Shimada, Mitsuo
AU - Sugimachi, Keizo
AU - Tachibana, Hirofumi
AU - Yamada, Koji
PY - 2001
Y1 - 2001
N2 - We examined the effect of dietary conjugated linoleic acid (CLA) on the growth of injected hepatoma dRLh-84 in Donryu rats. After experimental diets containing 0% or 2% CLA were given to male Donryu rats for 3 wk, dRLh-84 cells were injected into the left lobe of the hepatic capsule, and the experimental diet was continued. The cells formed a solid tumor ≥1 wk after the injection, and thereafter the tumor grew with feeding duration. In a morphological study, this tumor appeared to be a low-differentiated hepatoma, and there was no remarkable difference in the morphology of the tumor between 0% and 2% CLA groups. Tumor weight was significantly higher in the 2% CLA group than in the 0% CLA group throughout the feeding period after the injection. Serum glutamic-oxaloacetic transaminase and glutamic-pyruvic transaminase activities were significantly higher in 2% CLA-injected rats than in 0% CLA-injected rats at 3 wk after the injection. CLA upregulated acyl-CoA oxidase activity, especially 1 wk after the injection. However, dietary CLA did not activate carnitine palmitoyl transferase II, which is a rate-limiting enzyme in the mitochondrial β-oxidation pathway. Natural killer cell activity in the spleen tended to be higher in injected rats, but a significant effect of dietary CLA was not recognized. Serum interferon-γ and tumor necrosis factor-α levels were higher in injected than in sham rats. More-over, these levels were higher in 2% CLA groups than in the respective 0% CLA groups.
AB - We examined the effect of dietary conjugated linoleic acid (CLA) on the growth of injected hepatoma dRLh-84 in Donryu rats. After experimental diets containing 0% or 2% CLA were given to male Donryu rats for 3 wk, dRLh-84 cells were injected into the left lobe of the hepatic capsule, and the experimental diet was continued. The cells formed a solid tumor ≥1 wk after the injection, and thereafter the tumor grew with feeding duration. In a morphological study, this tumor appeared to be a low-differentiated hepatoma, and there was no remarkable difference in the morphology of the tumor between 0% and 2% CLA groups. Tumor weight was significantly higher in the 2% CLA group than in the 0% CLA group throughout the feeding period after the injection. Serum glutamic-oxaloacetic transaminase and glutamic-pyruvic transaminase activities were significantly higher in 2% CLA-injected rats than in 0% CLA-injected rats at 3 wk after the injection. CLA upregulated acyl-CoA oxidase activity, especially 1 wk after the injection. However, dietary CLA did not activate carnitine palmitoyl transferase II, which is a rate-limiting enzyme in the mitochondrial β-oxidation pathway. Natural killer cell activity in the spleen tended to be higher in injected rats, but a significant effect of dietary CLA was not recognized. Serum interferon-γ and tumor necrosis factor-α levels were higher in injected than in sham rats. More-over, these levels were higher in 2% CLA groups than in the respective 0% CLA groups.
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U2 - 10.1207/S15327914NC402_10
DO - 10.1207/S15327914NC402_10
M3 - Article
C2 - 11962249
AN - SCOPUS:0035729375
SN - 0163-5581
VL - 40
SP - 140
EP - 148
JO - Nutrition and Cancer
JF - Nutrition and Cancer
IS - 2
ER -
