TY - JOUR
T1 - Effect of diglucosamine on the entrapment of protein into liposomes
AU - Murakami, S.
AU - Ono, T.
AU - Sakai, S.
AU - Ijima, Hiroyuki
AU - Kawakami, K.
PY - 2006/7/1
Y1 - 2006/7/1
N2 - Liposomes, which had entrapped bovine serum albumin (BSA), were modified with diglucosamine by two methods. The liposome was prepared by a freeze-thawing method in the presence of the disaccharide, or the disaccharide was added to the liposome prepared in advance without it. To examine the effects of diglucosamine, the morphology, mean particle size, and zeta potential of both liposomes were compared with those of BSA-entrapping liposome prepared without the disaccharide. Diglucosamine caused no remarkable change in shape and no aggregation of the liposome. The presence of the disaccharide was confirmed on the surfaces of modified liposomes, and the entrapment of BSA into the liposomes was increased by the disaccharide. The entrapment behavior was affected by the way the disaccharide was added, and the difference in the way the BSA was entrapped was also indicated.
AB - Liposomes, which had entrapped bovine serum albumin (BSA), were modified with diglucosamine by two methods. The liposome was prepared by a freeze-thawing method in the presence of the disaccharide, or the disaccharide was added to the liposome prepared in advance without it. To examine the effects of diglucosamine, the morphology, mean particle size, and zeta potential of both liposomes were compared with those of BSA-entrapping liposome prepared without the disaccharide. Diglucosamine caused no remarkable change in shape and no aggregation of the liposome. The presence of the disaccharide was confirmed on the surfaces of modified liposomes, and the entrapment of BSA into the liposomes was increased by the disaccharide. The entrapment behavior was affected by the way the disaccharide was added, and the difference in the way the BSA was entrapped was also indicated.
UR - http://www.scopus.com/inward/record.url?scp=33744982085&partnerID=8YFLogxK
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U2 - 10.1080/08982100600680667
DO - 10.1080/08982100600680667
M3 - Article
C2 - 16753965
AN - SCOPUS:33744982085
VL - 16
SP - 103
EP - 112
JO - Journal of Liposome Research
JF - Journal of Liposome Research
SN - 0898-2104
IS - 2
ER -