Effect of dissolved oxygen concentration on monoclonal antibody production in hybridoma cell cultures

Tatsuya Ogawa, Masamichi Kamihira, Hitoshi Yoshida, Shinji Iijima, Takeshi Kobayashi

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

The effects of dissolved oxygen (DO) concentration on cell growth and antibody production in hybridoma cell cultivation were examined in batch culture with computer-controlled DO concentrations. In serum-containing medium, cell growth and antibody production were independent of DO value controlled in the range of 0.2-5 ppm. In serum-free medium, however, antibody production strongly depended on DO concentration for low DO values less than 2 ppm, in spite of the fact that specific growth rate did not vary greatly. Glucose consumption, lactate production and conversion to lactate increased with a decrease in DO in serum-free medium. On the other hand, conversion was high and independent of DO concentration in serum-containing medium. These results were observed in three hybridoma cell lines, including two mouse-mouse hybridomas and one human-human hybridoma.

Original languageEnglish
Pages (from-to)372-378
Number of pages7
JournalJournal of Fermentation and Bioengineering
Volume74
Issue number6
DOIs
Publication statusPublished - 1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

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