Effect of DNA length on dielectrophoretic characteristics of DNA-labeled microbeads

Hiromichi Kasahara, Zhenhao Ding, Michihiko Nakano, Junya Suehiro

Research output: Contribution to conferencePaper

3 Citations (Scopus)

Abstract

Polymerase chain reaction (PCR) is a powerful tool for diagnostic procedures in bacterial and viral infections. We have developed a new electrical technique for rapid detection of DNA amplified by PCR using dielectrophoresis (DEP) of microbeads that are chemically labeled with the amplicons. The DNA immobilization on the microbeads alters their DEP behavior in such a way that they are trapped on a microelectrode under the action of positive DEP, whereas pristine microbeads are not. Combining the dramatic alteration in DEP characteristics with impedance measurement leads to rapid and quantitative detection of amplicons. The method is based on the surface conductivity dependence of microbeads DEP characteristics. It was expected that the surface conductivity would depend on the length of DNA fragments immobilized on a microbeads. In this study, it was found that the crossover frequency was dependent on the length of DNA.

Original languageEnglish
Pages3341-3346
Number of pages6
DOIs
Publication statusPublished - Jun 16 2015
Event2015 IEEE International Conference on Industrial Technology, ICIT 2015 - Seville, Spain
Duration: Mar 17 2015Mar 19 2015

Other

Other2015 IEEE International Conference on Industrial Technology, ICIT 2015
CountrySpain
CitySeville
Period3/17/153/19/15

Fingerprint

Electrophoresis
DNA
Polymerase chain reaction
Microelectrodes

All Science Journal Classification (ASJC) codes

  • Computer Science Applications
  • Electrical and Electronic Engineering

Cite this

Kasahara, H., Ding, Z., Nakano, M., & Suehiro, J. (2015). Effect of DNA length on dielectrophoretic characteristics of DNA-labeled microbeads. 3341-3346. Paper presented at 2015 IEEE International Conference on Industrial Technology, ICIT 2015, Seville, Spain. https://doi.org/10.1109/ICIT.2015.7125593

Effect of DNA length on dielectrophoretic characteristics of DNA-labeled microbeads. / Kasahara, Hiromichi; Ding, Zhenhao; Nakano, Michihiko; Suehiro, Junya.

2015. 3341-3346 Paper presented at 2015 IEEE International Conference on Industrial Technology, ICIT 2015, Seville, Spain.

Research output: Contribution to conferencePaper

Kasahara, H, Ding, Z, Nakano, M & Suehiro, J 2015, 'Effect of DNA length on dielectrophoretic characteristics of DNA-labeled microbeads', Paper presented at 2015 IEEE International Conference on Industrial Technology, ICIT 2015, Seville, Spain, 3/17/15 - 3/19/15 pp. 3341-3346. https://doi.org/10.1109/ICIT.2015.7125593
Kasahara H, Ding Z, Nakano M, Suehiro J. Effect of DNA length on dielectrophoretic characteristics of DNA-labeled microbeads. 2015. Paper presented at 2015 IEEE International Conference on Industrial Technology, ICIT 2015, Seville, Spain. https://doi.org/10.1109/ICIT.2015.7125593
Kasahara, Hiromichi ; Ding, Zhenhao ; Nakano, Michihiko ; Suehiro, Junya. / Effect of DNA length on dielectrophoretic characteristics of DNA-labeled microbeads. Paper presented at 2015 IEEE International Conference on Industrial Technology, ICIT 2015, Seville, Spain.6 p.
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