Effect of DNA length on dielectrophoretic characteristics of DNA-labeled microbeads

Hiromichi Kasahara; Zhenhao Ding; Michihiko Nakano; Junya Suehiro

doi:10.1109/ICIT.2015.7125593

Effect of DNA length on dielectrophoretic characteristics of DNA-labeled microbeads

Hiromichi Kasahara, Zhenhao Ding, Michihiko Nakano, Junya Suehiro

Applied Energy Engineering

Research output: Contribution to conference › Paper › peer-review

4 Citations (Scopus)

Abstract

Polymerase chain reaction (PCR) is a powerful tool for diagnostic procedures in bacterial and viral infections. We have developed a new electrical technique for rapid detection of DNA amplified by PCR using dielectrophoresis (DEP) of microbeads that are chemically labeled with the amplicons. The DNA immobilization on the microbeads alters their DEP behavior in such a way that they are trapped on a microelectrode under the action of positive DEP, whereas pristine microbeads are not. Combining the dramatic alteration in DEP characteristics with impedance measurement leads to rapid and quantitative detection of amplicons. The method is based on the surface conductivity dependence of microbeads DEP characteristics. It was expected that the surface conductivity would depend on the length of DNA fragments immobilized on a microbeads. In this study, it was found that the crossover frequency was dependent on the length of DNA.

Original language	English
Pages	3341-3346
Number of pages	6
DOIs	https://doi.org/10.1109/ICIT.2015.7125593
Publication status	Published - Jun 16 2015
Event	2015 IEEE International Conference on Industrial Technology, ICIT 2015 - Seville, Spain Duration: Mar 17 2015 → Mar 19 2015

Other

Other	2015 IEEE International Conference on Industrial Technology, ICIT 2015
Country/Territory	Spain
City	Seville
Period	3/17/15 → 3/19/15

All Science Journal Classification (ASJC) codes

Computer Science Applications
Electrical and Electronic Engineering

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title = "Effect of DNA length on dielectrophoretic characteristics of DNA-labeled microbeads",

abstract = "Polymerase chain reaction (PCR) is a powerful tool for diagnostic procedures in bacterial and viral infections. We have developed a new electrical technique for rapid detection of DNA amplified by PCR using dielectrophoresis (DEP) of microbeads that are chemically labeled with the amplicons. The DNA immobilization on the microbeads alters their DEP behavior in such a way that they are trapped on a microelectrode under the action of positive DEP, whereas pristine microbeads are not. Combining the dramatic alteration in DEP characteristics with impedance measurement leads to rapid and quantitative detection of amplicons. The method is based on the surface conductivity dependence of microbeads DEP characteristics. It was expected that the surface conductivity would depend on the length of DNA fragments immobilized on a microbeads. In this study, it was found that the crossover frequency was dependent on the length of DNA.",

author = "Hiromichi Kasahara and Zhenhao Ding and Michihiko Nakano and Junya Suehiro",

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AU - Kasahara, Hiromichi

AU - Ding, Zhenhao

AU - Nakano, Michihiko

AU - Suehiro, Junya

PY - 2015/6/16

Y1 - 2015/6/16

N2 - Polymerase chain reaction (PCR) is a powerful tool for diagnostic procedures in bacterial and viral infections. We have developed a new electrical technique for rapid detection of DNA amplified by PCR using dielectrophoresis (DEP) of microbeads that are chemically labeled with the amplicons. The DNA immobilization on the microbeads alters their DEP behavior in such a way that they are trapped on a microelectrode under the action of positive DEP, whereas pristine microbeads are not. Combining the dramatic alteration in DEP characteristics with impedance measurement leads to rapid and quantitative detection of amplicons. The method is based on the surface conductivity dependence of microbeads DEP characteristics. It was expected that the surface conductivity would depend on the length of DNA fragments immobilized on a microbeads. In this study, it was found that the crossover frequency was dependent on the length of DNA.

AB - Polymerase chain reaction (PCR) is a powerful tool for diagnostic procedures in bacterial and viral infections. We have developed a new electrical technique for rapid detection of DNA amplified by PCR using dielectrophoresis (DEP) of microbeads that are chemically labeled with the amplicons. The DNA immobilization on the microbeads alters their DEP behavior in such a way that they are trapped on a microelectrode under the action of positive DEP, whereas pristine microbeads are not. Combining the dramatic alteration in DEP characteristics with impedance measurement leads to rapid and quantitative detection of amplicons. The method is based on the surface conductivity dependence of microbeads DEP characteristics. It was expected that the surface conductivity would depend on the length of DNA fragments immobilized on a microbeads. In this study, it was found that the crossover frequency was dependent on the length of DNA.

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Effect of DNA length on dielectrophoretic characteristics of DNA-labeled microbeads

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