Effect of EGFR and p-AKT Overexpression on Chromosomal Instability in Gastric Cancer

Yuichi Hisamatsu, Eiji Oki, Hajime Otsu, Koji Ando, Hiroshi Saeki, Eriko Tokunaga, Shinichi Aishima, Masaru Morita, Yoshinao Oda, Yoshihiko Maehara

Research output: Contribution to journalArticle

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Abstract

Background: Molecular profiling in gastric cancer (GC) is important for diagnosis and treatment. In this study, we investigated signal transduction pathways that might induce chromosomal instability in GC. Methods: Epidermal growth factor receptor (EGFR), human epidermal growth factor receptor 2 (HER2), and p-AKT expression were analyzed using immunohistochemistry, and chromosomal instability was assessed by DNA aneuploidy using laser scanning cytometry, in a total of 202 GC cases. Results: The rate of EGFR expression and p-AKT expression was 70.3 and 34.2 %, respectively, in GC patients. In total, 57.5 % of GC patients exhibited DNA aneuploidy, and p-AKT positively correlated with EGFR and HER2 (p = 0.0127 and p = 0.00031, respectively). Patients with EGFR overexpressing GC showed shorter disease-specific survival than the other cases (hazard ratio 2.00, 95 % confidence interval 1.19–3.53; p = 0.0104). Moreover, EGFR and p-AKT expression was significantly correlated with DNA aneuploidy (p = 0.0002 and p = 0.0302, respectively). Conclusions: Our data showed that both EGFR and p-AKT overexpression were clearly associated with DNA aneuploidy. Aneuploidy could be a useful marker for therapies that target EGFR.

Original languageEnglish
Pages (from-to)1986-1992
Number of pages7
JournalAnnals of Surgical Oncology
Volume23
Issue number6
DOIs
Publication statusPublished - Jun 1 2016

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Chromosomal Instability
Epidermal Growth Factor Receptor
Stomach Neoplasms
Aneuploidy
DNA
Laser Scanning Cytometry
Signal Transduction
Immunohistochemistry
Confidence Intervals
Survival
Therapeutics

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oncology

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Effect of EGFR and p-AKT Overexpression on Chromosomal Instability in Gastric Cancer. / Hisamatsu, Yuichi; Oki, Eiji; Otsu, Hajime; Ando, Koji; Saeki, Hiroshi; Tokunaga, Eriko; Aishima, Shinichi; Morita, Masaru; Oda, Yoshinao; Maehara, Yoshihiko.

In: Annals of Surgical Oncology, Vol. 23, No. 6, 01.06.2016, p. 1986-1992.

Research output: Contribution to journalArticle

Hisamatsu, Yuichi ; Oki, Eiji ; Otsu, Hajime ; Ando, Koji ; Saeki, Hiroshi ; Tokunaga, Eriko ; Aishima, Shinichi ; Morita, Masaru ; Oda, Yoshinao ; Maehara, Yoshihiko. / Effect of EGFR and p-AKT Overexpression on Chromosomal Instability in Gastric Cancer. In: Annals of Surgical Oncology. 2016 ; Vol. 23, No. 6. pp. 1986-1992.
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AU - Tokunaga, Eriko

AU - Aishima, Shinichi

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AB - Background: Molecular profiling in gastric cancer (GC) is important for diagnosis and treatment. In this study, we investigated signal transduction pathways that might induce chromosomal instability in GC. Methods: Epidermal growth factor receptor (EGFR), human epidermal growth factor receptor 2 (HER2), and p-AKT expression were analyzed using immunohistochemistry, and chromosomal instability was assessed by DNA aneuploidy using laser scanning cytometry, in a total of 202 GC cases. Results: The rate of EGFR expression and p-AKT expression was 70.3 and 34.2 %, respectively, in GC patients. In total, 57.5 % of GC patients exhibited DNA aneuploidy, and p-AKT positively correlated with EGFR and HER2 (p = 0.0127 and p = 0.00031, respectively). Patients with EGFR overexpressing GC showed shorter disease-specific survival than the other cases (hazard ratio 2.00, 95 % confidence interval 1.19–3.53; p = 0.0104). Moreover, EGFR and p-AKT expression was significantly correlated with DNA aneuploidy (p = 0.0002 and p = 0.0302, respectively). Conclusions: Our data showed that both EGFR and p-AKT overexpression were clearly associated with DNA aneuploidy. Aneuploidy could be a useful marker for therapies that target EGFR.

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