Effect of eicosapentaenoic acid on glucose-induced diacylglycerol synthesis in cultured bovine aortic endothelial cells

Tatsuya Kuroki, Toyoshi Inoguchi, Fumio Umeda, Hajime Nawata

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    Hyperglycemia has been postulated to increase diacylglycerol (DAG) level through de novo synthesis pathway and subsequently activate protein kinase C (PKC) in vascular cells, possibly leading to vascular dysfunction associated with diabetes. In this study, we examined the effect of eicosapentaenoic acid (EPA) on high glucose-induced increase in DAG level in cultured aortic endothelial cells (ECs). In ECs, total DAG level was significantly increased in the cells cultured with high glucose levels (400 mg/dl) compared with the cells with normal glucose levels (100 mg/dl). The addition of EPA completely prevented high glucose-induced increase in total DAG: level. In contrast, other common fatty acids such as palmitate and oleate significantly stimulated DAG syntheisis, although arachidonate did not affect it. High glucose level significantly stimulated the incorporation of 3 H-palmitate into DAG, while it did not affect the incorporation of 3 H-arachidonate into DAG. The addition of EPA completely prevented the high glucose-induced increase in 3 H-palmitate incorporation into DAG, while it did not affect the 3 H-arachidonate incorporation. These findings suggest that EPA can prevent high glucose induced-increase in DAG level in ECs, probably by specifically inhibiting de novo synthesis at the step of acylation. EPA may be one of the candidates for clinical agents normalizing activation of DAG-PKC pathway in diabetic vascular tissues and preventing vascular complications associated with diabetes.

    Original languageEnglish
    Pages (from-to)473-477
    Number of pages5
    JournalBiochemical and Biophysical Research Communications
    Volume247
    Issue number2
    DOIs
    Publication statusPublished - Jun 18 1998

    Fingerprint

    Eicosapentaenoic Acid
    Diglycerides
    Endothelial cells
    Endothelial Cells
    Glucose
    Blood Vessels
    Palmitates
    Medical problems
    Protein Kinase C
    Diacylglycerol Kinase
    Acylation
    Oleic Acid
    Hyperglycemia
    Cultured Cells
    Fatty Acids
    Chemical activation
    Tissue

    All Science Journal Classification (ASJC) codes

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Cite this

    Effect of eicosapentaenoic acid on glucose-induced diacylglycerol synthesis in cultured bovine aortic endothelial cells. / Kuroki, Tatsuya; Inoguchi, Toyoshi; Umeda, Fumio; Nawata, Hajime.

    In: Biochemical and Biophysical Research Communications, Vol. 247, No. 2, 18.06.1998, p. 473-477.

    Research output: Contribution to journalArticle

    Kuroki, Tatsuya ; Inoguchi, Toyoshi ; Umeda, Fumio ; Nawata, Hajime. / Effect of eicosapentaenoic acid on glucose-induced diacylglycerol synthesis in cultured bovine aortic endothelial cells. In: Biochemical and Biophysical Research Communications. 1998 ; Vol. 247, No. 2. pp. 473-477.
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