Effect of EPS1 gene deletion in Saccharomyces cerevisiae on the secretion of foreign proteins which have disulfide bridges

Jianwei He, Takashi Sakamoto, Youtao Song, Akira Saito, Akihito Harada, Hiroyuki Azakami, Akio Kato

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Both amyloid-prone cystatin and unstable mutant C94A lysozyme were secreted in wild-type and Δeps1 Saccharomyces cerevisiae cells. Amyloid-prone cystatin secreted at much higher level in Δeps1 cells than that in wild-type yeast. In parallel, the secretion amount of disulfide bond disrupted mutant C94A lysozyme greatly increased in Δeps1 cells although that was apparently low in wild-type yeast cells compared with the secretion amount of wild-type lysozyme. It is interesting that neither the unstable mutant C94A lysozyme nor amyloid-prone cystatin secreted in Δeps1 cells maintained their specific activities. These observations lead to the supposition that yeast cells deficient for the protein disulfide isomerase-family-member EPS1 locus secrete more of labile disulfide-containing model proteins.

Original languageEnglish
Pages (from-to)2277-2283
Number of pages7
JournalFEBS Letters
Volume579
Issue number11
DOIs
Publication statusPublished - Apr 25 2005

Fingerprint

Gene Deletion
Cystatins
Muramidase
Disulfides
Yeast
Saccharomyces cerevisiae
Genes
Amyloid
Proteins
Yeasts
Cells
Protein Disulfide-Isomerases

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Effect of EPS1 gene deletion in Saccharomyces cerevisiae on the secretion of foreign proteins which have disulfide bridges. / He, Jianwei; Sakamoto, Takashi; Song, Youtao; Saito, Akira; Harada, Akihito; Azakami, Hiroyuki; Kato, Akio.

In: FEBS Letters, Vol. 579, No. 11, 25.04.2005, p. 2277-2283.

Research output: Contribution to journalArticle

He, Jianwei ; Sakamoto, Takashi ; Song, Youtao ; Saito, Akira ; Harada, Akihito ; Azakami, Hiroyuki ; Kato, Akio. / Effect of EPS1 gene deletion in Saccharomyces cerevisiae on the secretion of foreign proteins which have disulfide bridges. In: FEBS Letters. 2005 ; Vol. 579, No. 11. pp. 2277-2283.
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AU - Kato, Akio

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