Effect of estrogens on the interferon-γ producing cell population of mouse splenocytes

Mako Nakaya, Hirofumi Tachibana, Koji Yamada

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We examined the effect of 17β-estradiol (E2) on the cytokine production by mouse splenocytes. The production of interferon-γ (IFN-γ) was enhanced by E2 stimulation. E2 increased the number of cells expressing IFN-γ and the IFN-γ mRNA expression level in the cells. There were low- and high-level cells expressing IFN-γ in the population. The natural killer (NK) cells and NKT cells were the low-level cells expressing IFN-γ, and the number of these cells was increased by E2 stimulation. In addition, it was suggested that the enhancing effect of IFN-γ production by E2 was mediated through estrogen receptor (ER) α, and ERβ agonist stimulated IFN-γ production. ERs are expressed on plasma membrane as well as in nucleus. The ligand specific to plasma membrane-associated ER (mER) enhanced the IFN-γ production. In conclusion, our results indicated that E2 up-regulated the IFN-γ production by mediating ERα, ERβ and mERs.

Original languageEnglish
Pages (from-to)47-53
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume70
Issue number1
DOIs
Publication statusPublished - Feb 2 2006

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Interferons
Estrogens
Cells
Population
Estrogen Receptors
Cell membranes
Cell Count
Cell Membrane
Natural Killer T-Cells
Natural Killer Cells
Estradiol
Cytokines
Ligands
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Effect of estrogens on the interferon-γ producing cell population of mouse splenocytes. / Nakaya, Mako; Tachibana, Hirofumi; Yamada, Koji.

In: Bioscience, Biotechnology and Biochemistry, Vol. 70, No. 1, 02.02.2006, p. 47-53.

Research output: Contribution to journalArticle

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