The effect of exogenous 5-aminolevulinic acid (ALA) on the production of extracellular heme peroxidases was investigated. The addition of ALA (2.0 mM) to white-rot fungi cultures increased lignin and manganese peroxidases (LiP and MnP) activities 5- and 10-fold, respectively, relative to the conventional ligninolytic cultures. However, the exogenous glycine and glutamate, biosynthetic precursors of ALA had no effect on LiP and MnP activities. It was also found that ALA did not act as a stimulator for LiP and MnP. A quantitative analysis revealed that ALA increased intracellular concentration of free heme. These results suggested that the rate determining step for fungal production of extracellular heme peroxidases is most likely ALA biosynthetic pathway.
|Publication status||Published - Jan 1 1998|
|Event||Proceedings of the 1998 7th International Conference on Biotechnology in the Pulp and Paper Industry. Part 1 (of 3) - Vancouver, Can|
Duration: Jun 16 1998 → Jun 19 1998
|Other||Proceedings of the 1998 7th International Conference on Biotechnology in the Pulp and Paper Industry. Part 1 (of 3)|
|Period||6/16/98 → 6/19/98|
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)