Effect of exogeneous 5-aminolevulinic acid on fungal production of extracellular heme peroxidases

Keita Matsushita, Hiroyuki Wariishi, Hiroo Tanaka

Research output: Contribution to conferencePaper

Abstract

The effect of exogenous 5-aminolevulinic acid (ALA) on the production of extracellular heme peroxidases was investigated. The addition of ALA (2.0 mM) to white-rot fungi cultures increased lignin and manganese peroxidases (LiP and MnP) activities 5- and 10-fold, respectively, relative to the conventional ligninolytic cultures. However, the exogenous glycine and glutamate, biosynthetic precursors of ALA had no effect on LiP and MnP activities. It was also found that ALA did not act as a stimulator for LiP and MnP. A quantitative analysis revealed that ALA increased intracellular concentration of free heme. These results suggested that the rate determining step for fungal production of extracellular heme peroxidases is most likely ALA biosynthetic pathway.

Original languageEnglish
Publication statusPublished - Jan 1 1998
EventProceedings of the 1998 7th International Conference on Biotechnology in the Pulp and Paper Industry. Part 1 (of 3) - Vancouver, Can
Duration: Jun 16 1998Jun 19 1998

Other

OtherProceedings of the 1998 7th International Conference on Biotechnology in the Pulp and Paper Industry. Part 1 (of 3)
CityVancouver, Can
Period6/16/986/19/98

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Peroxidases
Aminolevulinic Acid
Heme
Acids
manganese peroxidase
Biosynthetic Pathways
Lignin
Fungi
Glycine
Manganese
Amino acids
Glutamic Acid
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)

Cite this

Matsushita, K., Wariishi, H., & Tanaka, H. (1998). Effect of exogeneous 5-aminolevulinic acid on fungal production of extracellular heme peroxidases. Paper presented at Proceedings of the 1998 7th International Conference on Biotechnology in the Pulp and Paper Industry. Part 1 (of 3), Vancouver, Can, .

Effect of exogeneous 5-aminolevulinic acid on fungal production of extracellular heme peroxidases. / Matsushita, Keita; Wariishi, Hiroyuki; Tanaka, Hiroo.

1998. Paper presented at Proceedings of the 1998 7th International Conference on Biotechnology in the Pulp and Paper Industry. Part 1 (of 3), Vancouver, Can, .

Research output: Contribution to conferencePaper

Matsushita, K, Wariishi, H & Tanaka, H 1998, 'Effect of exogeneous 5-aminolevulinic acid on fungal production of extracellular heme peroxidases' Paper presented at Proceedings of the 1998 7th International Conference on Biotechnology in the Pulp and Paper Industry. Part 1 (of 3), Vancouver, Can, 6/16/98 - 6/19/98, .
Matsushita K, Wariishi H, Tanaka H. Effect of exogeneous 5-aminolevulinic acid on fungal production of extracellular heme peroxidases. 1998. Paper presented at Proceedings of the 1998 7th International Conference on Biotechnology in the Pulp and Paper Industry. Part 1 (of 3), Vancouver, Can, .
Matsushita, Keita ; Wariishi, Hiroyuki ; Tanaka, Hiroo. / Effect of exogeneous 5-aminolevulinic acid on fungal production of extracellular heme peroxidases. Paper presented at Proceedings of the 1998 7th International Conference on Biotechnology in the Pulp and Paper Industry. Part 1 (of 3), Vancouver, Can, .
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