TY - JOUR
T1 - Effect of extracellular ice formation and electrolyte concentration on injury of slowly frozen cells
AU - Takamatsu, Hiroshi
AU - Zawlodzka, Sylwia
AU - Miyanaga, Takeshi
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2006
Y1 - 2006
N2 - The mechanism of cell injury during slow freezing of cell suspension was examined by conducting two different kinds of experiments with PC-3 human prostate adenocarcinoma cells. One was a freezing experiment and the other was a pseudo-freezing experiment that exposed cells to a change in NaCl concentration with a synchronized change in the temperature, which mimicked the change in the environment of cells during freezing process. The results indicated that the mechanical stress from extracellular ice crystals played an important role in damaging cells during freezing at relatively higher freezing temperatures, while the solution effect that indicates the effect of increase in the solute concentration became more significant at lower freezing temperatures. A non-monotonous effect of temperature on cell injury due to the solution effect was also found, suggesting that the experimental results at the temperatures above 0°C could not be extrapolated to predict cell survival at sub-zero temperatures.
AB - The mechanism of cell injury during slow freezing of cell suspension was examined by conducting two different kinds of experiments with PC-3 human prostate adenocarcinoma cells. One was a freezing experiment and the other was a pseudo-freezing experiment that exposed cells to a change in NaCl concentration with a synchronized change in the temperature, which mimicked the change in the environment of cells during freezing process. The results indicated that the mechanical stress from extracellular ice crystals played an important role in damaging cells during freezing at relatively higher freezing temperatures, while the solution effect that indicates the effect of increase in the solute concentration became more significant at lower freezing temperatures. A non-monotonous effect of temperature on cell injury due to the solution effect was also found, suggesting that the experimental results at the temperatures above 0°C could not be extrapolated to predict cell survival at sub-zero temperatures.
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U2 - 10.1299/kikaib.72.1342
DO - 10.1299/kikaib.72.1342
M3 - Article
AN - SCOPUS:33746618303
VL - 72
SP - 1342
EP - 1348
JO - Nihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B
JF - Nihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B
SN - 0387-5016
IS - 5
ER -