TY - JOUR
T1 - Effect of fetal bovine serum concentration on lysophosphatidylcholine-mediated proliferation and apoptosis of human aortic smooth muscle cells
AU - Asai, Daisuke
AU - Kawano, Takahito
AU - Murata, Masaharu
AU - Nakashima, Hideki
AU - Toita, Riki
AU - Kang, Jeong Hun
N1 - Funding Information:
The authors thank Ms. Shigemi Terakubo and Ms. Niño Nakajim（a St. Marianna University School of Medicine）for technical assistance. This work was supported by JSPS KAKENHI grant numbers 17K08254, 17K01386, and 18K12091.
Publisher Copyright:
© 2020 by Japan Oil Chemists’ Society.
PY - 2020
Y1 - 2020
N2 - Lysophosphatidylcholine (lysoPtdCho) is produced by the phospholipase A2-mediated hydrolysis of phosphatidylcholine and can stimulate proliferation and apoptosis of vascular smooth muscle cells. We examined the influence of fetal bovine serum (FBS) concentration in the culture medium on lysoPtdCho-mediated apoptosis and proliferation of human aortic smooth muscle cells (HASMCs) as well as on the activation of extracellular signal-regulated kinases (ERK)1/2. In the presence of 1% FBS, HASMC viability increased after lysoPtdCho treatment at 1 and 10 μM but decreased at 25 and 50 μM. However, lysoPtdCho increased HASMC viability in a dose-dependent manner in the presence of 10% FBS. The activity of caspase 3/7 in HASMCs was increased by 25 μM lysoPtdCho in the presence of 1% FBS, but not 10% FBS. Furthermore, lysoPtdCho at 1 and 10 μM triggered ERK1/2 phosphorylation in the presence of 1% FBS, but not at 10% FBS. Thus, lysoPtdCho-mediated HASMC apoptosis, proliferation, and ERK1/2 activation are dependent on the concentration of FBS.
AB - Lysophosphatidylcholine (lysoPtdCho) is produced by the phospholipase A2-mediated hydrolysis of phosphatidylcholine and can stimulate proliferation and apoptosis of vascular smooth muscle cells. We examined the influence of fetal bovine serum (FBS) concentration in the culture medium on lysoPtdCho-mediated apoptosis and proliferation of human aortic smooth muscle cells (HASMCs) as well as on the activation of extracellular signal-regulated kinases (ERK)1/2. In the presence of 1% FBS, HASMC viability increased after lysoPtdCho treatment at 1 and 10 μM but decreased at 25 and 50 μM. However, lysoPtdCho increased HASMC viability in a dose-dependent manner in the presence of 10% FBS. The activity of caspase 3/7 in HASMCs was increased by 25 μM lysoPtdCho in the presence of 1% FBS, but not 10% FBS. Furthermore, lysoPtdCho at 1 and 10 μM triggered ERK1/2 phosphorylation in the presence of 1% FBS, but not at 10% FBS. Thus, lysoPtdCho-mediated HASMC apoptosis, proliferation, and ERK1/2 activation are dependent on the concentration of FBS.
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U2 - 10.5650/jos.ess19268
DO - 10.5650/jos.ess19268
M3 - Article
C2 - 32051357
AN - SCOPUS:85081037944
SN - 1345-8957
VL - 69
SP - 255
EP - 260
JO - Journal of Oleo Science
JF - Journal of Oleo Science
IS - 3
ER -