Effect of genetic background on Cav2 channel α1 and β subunit messenger RNA expression in cerebellum of N-type Ca 2+ channel α1B subunit-deficient mice

Eiki Takahashi, Mitsuhiro Ino, Takeshi Nagasu

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5 Citations (Scopus)

Abstract

Although the N-type Ca2+ channel plays a role in a variety of neuronal functions, N-type Ca2+ channel α1B- deficient mice exhibit normal life span without apparent behavioral or histologic abnormalities. To examine whether the reason for their normal behavior is compensation by other Cav2 channel α1 or β subunit genes and to analyze whether genetic background influences the subunit expression pattern, we studied the α1A, α1E, β1b, β2, β3 and β4 subunit mRNA levels in cerebellum of α1B-deficient mice with CBA x C57BL/6 or CBA/JN background. In cerebellum of the mice with a CBA x C57BL/6 background, α1A mRNA was expressed at a higher level than that in wild-type or heterozygous mice, but difference in the expression levels of α1E, β1b, β2, β3 and β4 subunits was not found among wild-type, heterozygous, and homozygous mice. In cerebellum of α1B-deficient mice with CBA/JN background, β4 mRNA was expressed at a higher level than that in wild-type or heterozygous mice, but α1A, α1E, β1b, α2 and β3 transcripts were expressed at similar levels in all genotypes. Therefore, a possible explanation of the normal behavior of α1B-deficient mice is that Cav2 channel family members compensate for the deficiency, and that the change of functional subunit expression pattern for compensation differs in animals with different genetic backgrounds.

Original languageEnglish
Pages (from-to)690-694
Number of pages5
JournalComparative Medicine
Volume54
Issue number6
Publication statusPublished - Dec 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

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