Effect of glucose on Na, K-ATPase activity in cultured bovine aortic endothelial cells

T. Yamashita, F. Umeda, T. Hashimoto, T. Inoguchi, T. Yamauchi, K. Mimura, J. Watanabe, H. Nawata

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    Abstract

    The effect of high concentrations of glucose on Na, K-ATPase activity and the polyol pathway was studied using cultured bovine aortic endothelial cells. Na, K-ATPase activity was expressed as ouabain-sensitive K+ uptake. A significant decrease in Na, K-ATPase activity with an intracellular accumulation of sorbitol was found in confluent endothelial cells incubated with 400 mg/dl glucose for 96 h. However, there was no significant change in the Na, K-ATPase activity or sorbitol content of the cells incubated with 100 mg/dl glucose plus 300 mg/dl mannitol. The decrease in Na, K-ATPase induced by the high glucose concentration was restored by the simultaneous addition of 10-4M ponalrestat (ICI 128,436; Statil), an aldose reductase inhibitor. The addition of this agent also significantly reduced the increase in sorbitol induced by high glucose levels. These results suggest that the decrease in Na, K-ATPase activity induced in cultured aortic endothelial cells by high concentrations of glucose may be caused in part by the accumulation of sorbitol.

    Original languageEnglish
    Pages (from-to)1-7
    Number of pages7
    JournalEndocrinologia Japonica
    Volume39
    Issue number1
    DOIs
    Publication statusPublished - Jan 1 1992

    Fingerprint

    Endothelial cells
    Glucose
    Endothelial Cells
    Sorbitol
    Aldehyde Reductase
    Polyols
    Mannitol
    Ouabain
    sodium-translocating ATPase
    Adenosine Triphosphatases

    All Science Journal Classification (ASJC) codes

    • Endocrinology
    • Engineering(all)

    Cite this

    Yamashita, T., Umeda, F., Hashimoto, T., Inoguchi, T., Yamauchi, T., Mimura, K., ... Nawata, H. (1992). Effect of glucose on Na, K-ATPase activity in cultured bovine aortic endothelial cells. Endocrinologia Japonica, 39(1), 1-7. https://doi.org/10.1507/endocrj1954.39.1

    Effect of glucose on Na, K-ATPase activity in cultured bovine aortic endothelial cells. / Yamashita, T.; Umeda, F.; Hashimoto, T.; Inoguchi, T.; Yamauchi, T.; Mimura, K.; Watanabe, J.; Nawata, H.

    In: Endocrinologia Japonica, Vol. 39, No. 1, 01.01.1992, p. 1-7.

    Research output: Contribution to journalArticle

    Yamashita, T, Umeda, F, Hashimoto, T, Inoguchi, T, Yamauchi, T, Mimura, K, Watanabe, J & Nawata, H 1992, 'Effect of glucose on Na, K-ATPase activity in cultured bovine aortic endothelial cells', Endocrinologia Japonica, vol. 39, no. 1, pp. 1-7. https://doi.org/10.1507/endocrj1954.39.1
    Yamashita T, Umeda F, Hashimoto T, Inoguchi T, Yamauchi T, Mimura K et al. Effect of glucose on Na, K-ATPase activity in cultured bovine aortic endothelial cells. Endocrinologia Japonica. 1992 Jan 1;39(1):1-7. https://doi.org/10.1507/endocrj1954.39.1
    Yamashita, T. ; Umeda, F. ; Hashimoto, T. ; Inoguchi, T. ; Yamauchi, T. ; Mimura, K. ; Watanabe, J. ; Nawata, H. / Effect of glucose on Na, K-ATPase activity in cultured bovine aortic endothelial cells. In: Endocrinologia Japonica. 1992 ; Vol. 39, No. 1. pp. 1-7.
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