Effect of high pressure on the accumulation of IMP and on the stability of AMP deaminase in rabbit skeletal muscle

Sunao Mori, Akiko Uchida, Shuhei Yamamoto, Asma Sultana, Ryuichi Tatsumi, Wataru Mizunoya, Atsushi Suzuki, Yoshihide Ikeuchi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This study evaluated the effect of high pressure on rabbit skeletal muscle, specifically on the production of inosinic acid (IMP), one of "umami" components, and on the activity of adenosine triphosphate (AMP) deaminase, which plays a role in the conversion of AMP to IMP. By increasing the pressure (0.1 to 300 MPa), nucleotide analysis showed that IMP content in muscle increased instantly with a concomitant decrease in ATP content. The IMP content of muscle at 300 MPa was approximately 15% higher than with lower pressures (0.1-200 MPa) when stored for 1 week at 4C after pressurization. These results suggested that the metabolism of nucleotides in muscle was not significantly impaired by pressure treatment. At 300 MPa, AMP deaminase maintained approximately 70% of the activity at 0.1 MPa. In contrast, the activity of purified AMP deaminase was completely lost at 200 MPa, and irreversible conformational changes were observed by in situ fluorescence spectroscopy. These results indicated that purified AMP deaminase was irreversibly denatured under pressure as high as 300-400 MPa.

Original languageEnglish
Pages (from-to)328-342
Number of pages15
JournalJournal of Food Biochemistry
Volume31
Issue number3
DOIs
Publication statusPublished - Jun 1 2007

Fingerprint

AMP deaminase
AMP Deaminase
Inosine Monophosphate
Muscle
skeletal muscle
Skeletal Muscle
rabbits
Rabbits
Pressure
muscles
fluorescence emission spectroscopy
Muscles
nucleotides
umami
Nucleotides
Adenosine Triphosphate
pressure treatment
adenosine triphosphate
Pressurization
Fluorescence Spectrometry

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Food Science
  • Pharmacology
  • Cell Biology

Cite this

Effect of high pressure on the accumulation of IMP and on the stability of AMP deaminase in rabbit skeletal muscle. / Mori, Sunao; Uchida, Akiko; Yamamoto, Shuhei; Sultana, Asma; Tatsumi, Ryuichi; Mizunoya, Wataru; Suzuki, Atsushi; Ikeuchi, Yoshihide.

In: Journal of Food Biochemistry, Vol. 31, No. 3, 01.06.2007, p. 328-342.

Research output: Contribution to journalArticle

Mori, Sunao ; Uchida, Akiko ; Yamamoto, Shuhei ; Sultana, Asma ; Tatsumi, Ryuichi ; Mizunoya, Wataru ; Suzuki, Atsushi ; Ikeuchi, Yoshihide. / Effect of high pressure on the accumulation of IMP and on the stability of AMP deaminase in rabbit skeletal muscle. In: Journal of Food Biochemistry. 2007 ; Vol. 31, No. 3. pp. 328-342.
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