Effect of high pressure treatment on cytoplasmic 5′-nucleotidase from rabbit skeletal muscle

Sunao Mori, Atsuko Yokoyama, Reiko Iguchi, Shuhei Yamamoto, Atsushi Suzuki, Wataru Mizunoya, Ryuichi Tatsumi, Keiko Yoshioka, Yoshihide Ikeuchi

Research output: Contribution to journalArticle

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Abstract

We investigated the effect of high-pressure treatment on the properties of cytoplasmic 5′-nucleotidase (NT), which converts inosine monophosphate (IMP) into inosine. After pressure treatment at 400 MPa, the activity of purified IMP-NT remained at almost 100%, but the activity of partially purified adenosine monophosphate (AMP)-NT decreased to about 40%. These data suggest that there is a difference in the pressure stability between the enzymes. In situ fluorescence spectroscopy of IMP-NT under pressure showed that its pressure-induced denaturation was reversible. When the pressure was reduced from the highest pressure to ambient pressure, hysteresis was observed. This suggests that high pressure treatment may lead to a partial change in the affinity of the subunits for each other once they have dissociated. The activities of IMP-NT and AMP-NT extracted from pressure-treated muscles decreased remarkably between 250 and 450 MPa, but IMP-NT was more stable than AMP-NT.

Original languageEnglish
Pages (from-to)314-327
Number of pages14
JournalJournal of Food Biochemistry
Volume31
Issue number3
DOIs
Publication statusPublished - Jun 1 2007

Fingerprint

nucleotidase
5'-nucleotidase
5'-Nucleotidase
high pressure treatment
inosine monophosphate
skeletal muscle
Skeletal Muscle
rabbits
Rabbits
Pressure
Inosine Monophosphate
adenosine monophosphate
Adenosine Monophosphate
fluorescence emission spectroscopy
pressure treatment
hysteresis
denaturation
Enzyme Stability
Inosine
Fluorescence Spectrometry

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Food Science
  • Pharmacology
  • Cell Biology

Cite this

Mori, S., Yokoyama, A., Iguchi, R., Yamamoto, S., Suzuki, A., Mizunoya, W., ... Ikeuchi, Y. (2007). Effect of high pressure treatment on cytoplasmic 5′-nucleotidase from rabbit skeletal muscle. Journal of Food Biochemistry, 31(3), 314-327. https://doi.org/10.1111/j.1745-4514.2007.00117.x

Effect of high pressure treatment on cytoplasmic 5′-nucleotidase from rabbit skeletal muscle. / Mori, Sunao; Yokoyama, Atsuko; Iguchi, Reiko; Yamamoto, Shuhei; Suzuki, Atsushi; Mizunoya, Wataru; Tatsumi, Ryuichi; Yoshioka, Keiko; Ikeuchi, Yoshihide.

In: Journal of Food Biochemistry, Vol. 31, No. 3, 01.06.2007, p. 314-327.

Research output: Contribution to journalArticle

Mori, S, Yokoyama, A, Iguchi, R, Yamamoto, S, Suzuki, A, Mizunoya, W, Tatsumi, R, Yoshioka, K & Ikeuchi, Y 2007, 'Effect of high pressure treatment on cytoplasmic 5′-nucleotidase from rabbit skeletal muscle', Journal of Food Biochemistry, vol. 31, no. 3, pp. 314-327. https://doi.org/10.1111/j.1745-4514.2007.00117.x
Mori, Sunao ; Yokoyama, Atsuko ; Iguchi, Reiko ; Yamamoto, Shuhei ; Suzuki, Atsushi ; Mizunoya, Wataru ; Tatsumi, Ryuichi ; Yoshioka, Keiko ; Ikeuchi, Yoshihide. / Effect of high pressure treatment on cytoplasmic 5′-nucleotidase from rabbit skeletal muscle. In: Journal of Food Biochemistry. 2007 ; Vol. 31, No. 3. pp. 314-327.
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