Effect of high pressure treatment on cytoplasmic 5′-nucleotidase from rabbit skeletal muscle

Sunao Mori, Atsuko Yokoyama, Reiko Iguchi, Shuhei Yamamoto, Atsushi Suzuki, Wataru Mizunoya, Ryuichi Tatsumi, Keiko Yoshioka, Yoshihide Ikeuchi

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5 Citations (Scopus)

Abstract

We investigated the effect of high-pressure treatment on the properties of cytoplasmic 5′-nucleotidase (NT), which converts inosine monophosphate (IMP) into inosine. After pressure treatment at 400 MPa, the activity of purified IMP-NT remained at almost 100%, but the activity of partially purified adenosine monophosphate (AMP)-NT decreased to about 40%. These data suggest that there is a difference in the pressure stability between the enzymes. In situ fluorescence spectroscopy of IMP-NT under pressure showed that its pressure-induced denaturation was reversible. When the pressure was reduced from the highest pressure to ambient pressure, hysteresis was observed. This suggests that high pressure treatment may lead to a partial change in the affinity of the subunits for each other once they have dissociated. The activities of IMP-NT and AMP-NT extracted from pressure-treated muscles decreased remarkably between 250 and 450 MPa, but IMP-NT was more stable than AMP-NT.

Original languageEnglish
Pages (from-to)314-327
Number of pages14
JournalJournal of Food Biochemistry
Volume31
Issue number3
DOIs
Publication statusPublished - Jun 1 2007

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Food Science
  • Pharmacology
  • Cell Biology

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