Effect of honeycomb-patterned surface topography on the adhesion and signal transduction of porcine aortic endothelial cells

S. Yamamoto, Masaru Tanaka, H. Sunami, E. Ito, S. Yamashita, Y. Morita, M. Shimomura

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Surface topography has vital roles in cellular response. Here, to investigate the mechanism behind cellular response to surface topography, we prepared honeycomb (HC)-patterned films from poly(ε-caprolactone) (PCL) with micropatterned surface topography by casting a polymer solution of water-immiscible solvent under high humidity. We characterized the adsorption of fibronectin (Fn) on the film using atomic force microscopy (AFM) and confocal laser scanning microscopy (CLSM). The response of porcine aortic endothelial cells (PAECs) to adsorbed Fn molecules onto HC-patterned films was observed by immunofluorescence labeling of vinculin and the actin fiber of PAECs cultured for 1 and 72 h in serum-free medium. The expression of focal adhesion kinase autophosphorylated at the tyrosine residue (pFAK) at 1 h culture was determined using an immunoprecipitation method. Fn adsorbed selectively around the pore edges to form ring-shaped aggregates. The immunostaining results revealed that PAECs adhered to the HC-patterned films at focal contact points localized around pore peripheries. These points correspond to adsorption sites of Fn. The expression of pFAK after 1 h on the HC-patterned film was 3 times higher than that on a corresponding flat film, indicating that the signaling mediated by the binding between Fn and the integrin receptor was more highly activated on the HC-patterned film. These results suggest that the cellular response to HC-patterned films (e.g., adhesion pattern and phosphorylation of FAK) originates from the regularly aligned adsorption pattern of Fn determined by the pore structure of the film.

Original languageEnglish
Pages (from-to)8114-8120
Number of pages7
JournalLangmuir
Volume23
Issue number15
DOIs
Publication statusPublished - Jul 17 2007
Externally publishedYes

Fingerprint

Signal transduction
Endothelial cells
Surface topography
topography
adhesion
Adhesion
Fibronectins
porosity
Adsorption
adsorption
Vinculin
Focal Adhesion Protein-Tyrosine Kinases
phosphorylation
Phosphorylation
tyrosine
Serum-Free Culture Media
Point contacts
Polymer solutions
Pore structure
cultured cells

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Effect of honeycomb-patterned surface topography on the adhesion and signal transduction of porcine aortic endothelial cells. / Yamamoto, S.; Tanaka, Masaru; Sunami, H.; Ito, E.; Yamashita, S.; Morita, Y.; Shimomura, M.

In: Langmuir, Vol. 23, No. 15, 17.07.2007, p. 8114-8120.

Research output: Contribution to journalArticle

Yamamoto, S. ; Tanaka, Masaru ; Sunami, H. ; Ito, E. ; Yamashita, S. ; Morita, Y. ; Shimomura, M. / Effect of honeycomb-patterned surface topography on the adhesion and signal transduction of porcine aortic endothelial cells. In: Langmuir. 2007 ; Vol. 23, No. 15. pp. 8114-8120.
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