Effect of intramammary infusion of recombinant bovine GM-CSF and IL-8 on CMT score, somatic cell count, and milk mononuclear cell populations in Holstein cows with Staphylococcus aureus subclinical mastitis

Yoshio Kiku, Tomomi Ozawa, Hideyuki Takahashi, Shiro Kushibiki, Shigeki Inumaru, Hiroyuki Shingu, Yuya Nagasawa, Atsushi Watanabe, Eiji Hata, Tomohito Hayashi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The effect of intramammary infusion of recombinant bovine granulocyte-macrophage colony-stimulating factor (rbGM-CSF) and interleukin-8 (rbIL-8) on mononuclear cell populations in quarters, somatic cell count (SCC) and the California Mastitis Test (CMT) score were investigated. From the selected cows with naturally occurring Staphylococcus aureus subclinical mastitis, one quarter of each cow were selected for the infusions of rbGM-CSF (400 μg/5 mL/quarter, n = 9), rbIL-8 (1 mg/5 mL/quarter, n = 9), and phosphate-buffered saline (5 mL/quarter, n = 7). The CMT score of both cytokines post infusion temporarily increased between days 0 and 1 and significantly decreased between days 7 and 14 compared to the preinfusion level. The SCC on day 14 after infusions of rbGM-CSF tended to be lower than that of the control group. The percentage of CD14+ cells increased on days 1 and 2 post infusion of rbGM-CSF. The percentage of CD4+ and CD8+ cells also increased on days 2 and 3, suggesting that the infusion of rbGM-CSF enhanced cellular immunity in the mammary gland. In contrast, the percentage of CD14+ cells decreased on days 0.25 and 1 post infusion of rbIL-8. No significant changes in the percentages of CD4+ and CD8+ cells in milk after infusion of rbIL-8 were evident during the experimental period, which suggested that rbIL-8 had little effect on the function of T cells in the mammary gland. These results indicated that rbGM-CSF and rbIL-8 decreased the CMT score by a different mechanism and may have a potential as therapeutic agents for subclinical mastitis.

Original languageEnglish
Pages (from-to)175-182
Number of pages8
JournalVeterinary Research Communications
Volume41
Issue number3
DOIs
Publication statusPublished - Sep 1 2017
Externally publishedYes

Fingerprint

granulocyte-macrophage colony-stimulating factor
Mastitis
interleukin-8
Granulocyte-Macrophage Colony-Stimulating Factor
somatic cell count
Interleukin-8
mastitis
Staphylococcus aureus
Milk
Holstein
Cell Count
cows
milk
cattle
Population
cells
Human Mammary Glands
mammary glands
Cellular Immunity
cell-mediated immunity

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

Effect of intramammary infusion of recombinant bovine GM-CSF and IL-8 on CMT score, somatic cell count, and milk mononuclear cell populations in Holstein cows with Staphylococcus aureus subclinical mastitis. / Kiku, Yoshio; Ozawa, Tomomi; Takahashi, Hideyuki; Kushibiki, Shiro; Inumaru, Shigeki; Shingu, Hiroyuki; Nagasawa, Yuya; Watanabe, Atsushi; Hata, Eiji; Hayashi, Tomohito.

In: Veterinary Research Communications, Vol. 41, No. 3, 01.09.2017, p. 175-182.

Research output: Contribution to journalArticle

Kiku, Yoshio ; Ozawa, Tomomi ; Takahashi, Hideyuki ; Kushibiki, Shiro ; Inumaru, Shigeki ; Shingu, Hiroyuki ; Nagasawa, Yuya ; Watanabe, Atsushi ; Hata, Eiji ; Hayashi, Tomohito. / Effect of intramammary infusion of recombinant bovine GM-CSF and IL-8 on CMT score, somatic cell count, and milk mononuclear cell populations in Holstein cows with Staphylococcus aureus subclinical mastitis. In: Veterinary Research Communications. 2017 ; Vol. 41, No. 3. pp. 175-182.
@article{655b1b5b8d9546c2bb62c90eb86a2c5f,
title = "Effect of intramammary infusion of recombinant bovine GM-CSF and IL-8 on CMT score, somatic cell count, and milk mononuclear cell populations in Holstein cows with Staphylococcus aureus subclinical mastitis",
abstract = "The effect of intramammary infusion of recombinant bovine granulocyte-macrophage colony-stimulating factor (rbGM-CSF) and interleukin-8 (rbIL-8) on mononuclear cell populations in quarters, somatic cell count (SCC) and the California Mastitis Test (CMT) score were investigated. From the selected cows with naturally occurring Staphylococcus aureus subclinical mastitis, one quarter of each cow were selected for the infusions of rbGM-CSF (400 μg/5 mL/quarter, n = 9), rbIL-8 (1 mg/5 mL/quarter, n = 9), and phosphate-buffered saline (5 mL/quarter, n = 7). The CMT score of both cytokines post infusion temporarily increased between days 0 and 1 and significantly decreased between days 7 and 14 compared to the preinfusion level. The SCC on day 14 after infusions of rbGM-CSF tended to be lower than that of the control group. The percentage of CD14+ cells increased on days 1 and 2 post infusion of rbGM-CSF. The percentage of CD4+ and CD8+ cells also increased on days 2 and 3, suggesting that the infusion of rbGM-CSF enhanced cellular immunity in the mammary gland. In contrast, the percentage of CD14+ cells decreased on days 0.25 and 1 post infusion of rbIL-8. No significant changes in the percentages of CD4+ and CD8+ cells in milk after infusion of rbIL-8 were evident during the experimental period, which suggested that rbIL-8 had little effect on the function of T cells in the mammary gland. These results indicated that rbGM-CSF and rbIL-8 decreased the CMT score by a different mechanism and may have a potential as therapeutic agents for subclinical mastitis.",
author = "Yoshio Kiku and Tomomi Ozawa and Hideyuki Takahashi and Shiro Kushibiki and Shigeki Inumaru and Hiroyuki Shingu and Yuya Nagasawa and Atsushi Watanabe and Eiji Hata and Tomohito Hayashi",
year = "2017",
month = "9",
day = "1",
doi = "10.1007/s11259-017-9684-y",
language = "English",
volume = "41",
pages = "175--182",
journal = "Veterinary Research Communications",
issn = "0165-7380",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - Effect of intramammary infusion of recombinant bovine GM-CSF and IL-8 on CMT score, somatic cell count, and milk mononuclear cell populations in Holstein cows with Staphylococcus aureus subclinical mastitis

AU - Kiku, Yoshio

AU - Ozawa, Tomomi

AU - Takahashi, Hideyuki

AU - Kushibiki, Shiro

AU - Inumaru, Shigeki

AU - Shingu, Hiroyuki

AU - Nagasawa, Yuya

AU - Watanabe, Atsushi

AU - Hata, Eiji

AU - Hayashi, Tomohito

PY - 2017/9/1

Y1 - 2017/9/1

N2 - The effect of intramammary infusion of recombinant bovine granulocyte-macrophage colony-stimulating factor (rbGM-CSF) and interleukin-8 (rbIL-8) on mononuclear cell populations in quarters, somatic cell count (SCC) and the California Mastitis Test (CMT) score were investigated. From the selected cows with naturally occurring Staphylococcus aureus subclinical mastitis, one quarter of each cow were selected for the infusions of rbGM-CSF (400 μg/5 mL/quarter, n = 9), rbIL-8 (1 mg/5 mL/quarter, n = 9), and phosphate-buffered saline (5 mL/quarter, n = 7). The CMT score of both cytokines post infusion temporarily increased between days 0 and 1 and significantly decreased between days 7 and 14 compared to the preinfusion level. The SCC on day 14 after infusions of rbGM-CSF tended to be lower than that of the control group. The percentage of CD14+ cells increased on days 1 and 2 post infusion of rbGM-CSF. The percentage of CD4+ and CD8+ cells also increased on days 2 and 3, suggesting that the infusion of rbGM-CSF enhanced cellular immunity in the mammary gland. In contrast, the percentage of CD14+ cells decreased on days 0.25 and 1 post infusion of rbIL-8. No significant changes in the percentages of CD4+ and CD8+ cells in milk after infusion of rbIL-8 were evident during the experimental period, which suggested that rbIL-8 had little effect on the function of T cells in the mammary gland. These results indicated that rbGM-CSF and rbIL-8 decreased the CMT score by a different mechanism and may have a potential as therapeutic agents for subclinical mastitis.

AB - The effect of intramammary infusion of recombinant bovine granulocyte-macrophage colony-stimulating factor (rbGM-CSF) and interleukin-8 (rbIL-8) on mononuclear cell populations in quarters, somatic cell count (SCC) and the California Mastitis Test (CMT) score were investigated. From the selected cows with naturally occurring Staphylococcus aureus subclinical mastitis, one quarter of each cow were selected for the infusions of rbGM-CSF (400 μg/5 mL/quarter, n = 9), rbIL-8 (1 mg/5 mL/quarter, n = 9), and phosphate-buffered saline (5 mL/quarter, n = 7). The CMT score of both cytokines post infusion temporarily increased between days 0 and 1 and significantly decreased between days 7 and 14 compared to the preinfusion level. The SCC on day 14 after infusions of rbGM-CSF tended to be lower than that of the control group. The percentage of CD14+ cells increased on days 1 and 2 post infusion of rbGM-CSF. The percentage of CD4+ and CD8+ cells also increased on days 2 and 3, suggesting that the infusion of rbGM-CSF enhanced cellular immunity in the mammary gland. In contrast, the percentage of CD14+ cells decreased on days 0.25 and 1 post infusion of rbIL-8. No significant changes in the percentages of CD4+ and CD8+ cells in milk after infusion of rbIL-8 were evident during the experimental period, which suggested that rbIL-8 had little effect on the function of T cells in the mammary gland. These results indicated that rbGM-CSF and rbIL-8 decreased the CMT score by a different mechanism and may have a potential as therapeutic agents for subclinical mastitis.

UR - http://www.scopus.com/inward/record.url?scp=85014586220&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85014586220&partnerID=8YFLogxK

U2 - 10.1007/s11259-017-9684-y

DO - 10.1007/s11259-017-9684-y

M3 - Article

C2 - 28281038

AN - SCOPUS:85014586220

VL - 41

SP - 175

EP - 182

JO - Veterinary Research Communications

JF - Veterinary Research Communications

SN - 0165-7380

IS - 3

ER -