Effect of irreversible electroporation on three-dimensional cell culture model

Kosaku Kurata, Masahiro Matsushita, Takashi Yoshii, Takanobu Fukunaga, Hiroshi Takamatsu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Irreversible electroporation (IRE) is a new treatment to necrotize abnormal cells by high electric pulses. Electric potential difference over 1 V across the plasma membrane permanently permeabilizes the cell with keeping the extracellular matrix intact if the thermal damage due to the Joule heating effect is avoided. This is the largest advantage of the IRE compared to the other conventional treatment. However, since the IRE has just started to be used in clinical tests, it is important to predict the necrotized region that depends on pulse parameters and electrode arrangement. We therefore examined the numerical solution to the Laplace equation for the static electric field to predict the IRE-induced cell necrosis. Three-dimensionally (3-D) cultured cells in a tissue phantom were experimentally subjected to the electric pulses through a pair of puncture electrodes. The necrotized area was determined as a function of the pulse repetition and compared with the area that was estimated by the numerical analysis.

Original languageEnglish
Title of host publication2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2012
Pages179-182
Number of pages4
DOIs
Publication statusPublished - Dec 14 2012
Event34th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2012 - San Diego, CA, United States
Duration: Aug 28 2012Sep 1 2012

Publication series

NameProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN (Print)1557-170X

Other

Other34th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2012
CountryUnited States
CitySan Diego, CA
Period8/28/129/1/12

Fingerprint

Electroporation
Cell culture
Cell Culture Techniques
Electrodes
Joule heating
Laplace equation
Cell membranes
Numerical analysis
Cells
Electric fields
Tissue
Electric potential
Punctures
Heating
Extracellular Matrix
Cultured Cells
Necrosis
Hot Temperature
Cell Membrane
Therapeutics

All Science Journal Classification (ASJC) codes

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

Cite this

Kurata, K., Matsushita, M., Yoshii, T., Fukunaga, T., & Takamatsu, H. (2012). Effect of irreversible electroporation on three-dimensional cell culture model. In 2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2012 (pp. 179-182). [6345900] (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). https://doi.org/10.1109/EMBC.2012.6345900

Effect of irreversible electroporation on three-dimensional cell culture model. / Kurata, Kosaku; Matsushita, Masahiro; Yoshii, Takashi; Fukunaga, Takanobu; Takamatsu, Hiroshi.

2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2012. 2012. p. 179-182 6345900 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kurata, K, Matsushita, M, Yoshii, T, Fukunaga, T & Takamatsu, H 2012, Effect of irreversible electroporation on three-dimensional cell culture model. in 2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2012., 6345900, Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS, pp. 179-182, 34th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2012, San Diego, CA, United States, 8/28/12. https://doi.org/10.1109/EMBC.2012.6345900
Kurata K, Matsushita M, Yoshii T, Fukunaga T, Takamatsu H. Effect of irreversible electroporation on three-dimensional cell culture model. In 2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2012. 2012. p. 179-182. 6345900. (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). https://doi.org/10.1109/EMBC.2012.6345900
Kurata, Kosaku ; Matsushita, Masahiro ; Yoshii, Takashi ; Fukunaga, Takanobu ; Takamatsu, Hiroshi. / Effect of irreversible electroporation on three-dimensional cell culture model. 2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2012. 2012. pp. 179-182 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).
@inproceedings{2c40164509a048de811c6471dde0dfe6,
title = "Effect of irreversible electroporation on three-dimensional cell culture model",
abstract = "Irreversible electroporation (IRE) is a new treatment to necrotize abnormal cells by high electric pulses. Electric potential difference over 1 V across the plasma membrane permanently permeabilizes the cell with keeping the extracellular matrix intact if the thermal damage due to the Joule heating effect is avoided. This is the largest advantage of the IRE compared to the other conventional treatment. However, since the IRE has just started to be used in clinical tests, it is important to predict the necrotized region that depends on pulse parameters and electrode arrangement. We therefore examined the numerical solution to the Laplace equation for the static electric field to predict the IRE-induced cell necrosis. Three-dimensionally (3-D) cultured cells in a tissue phantom were experimentally subjected to the electric pulses through a pair of puncture electrodes. The necrotized area was determined as a function of the pulse repetition and compared with the area that was estimated by the numerical analysis.",
author = "Kosaku Kurata and Masahiro Matsushita and Takashi Yoshii and Takanobu Fukunaga and Hiroshi Takamatsu",
year = "2012",
month = "12",
day = "14",
doi = "10.1109/EMBC.2012.6345900",
language = "English",
isbn = "9781424441198",
series = "Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS",
pages = "179--182",
booktitle = "2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2012",

}

TY - GEN

T1 - Effect of irreversible electroporation on three-dimensional cell culture model

AU - Kurata, Kosaku

AU - Matsushita, Masahiro

AU - Yoshii, Takashi

AU - Fukunaga, Takanobu

AU - Takamatsu, Hiroshi

PY - 2012/12/14

Y1 - 2012/12/14

N2 - Irreversible electroporation (IRE) is a new treatment to necrotize abnormal cells by high electric pulses. Electric potential difference over 1 V across the plasma membrane permanently permeabilizes the cell with keeping the extracellular matrix intact if the thermal damage due to the Joule heating effect is avoided. This is the largest advantage of the IRE compared to the other conventional treatment. However, since the IRE has just started to be used in clinical tests, it is important to predict the necrotized region that depends on pulse parameters and electrode arrangement. We therefore examined the numerical solution to the Laplace equation for the static electric field to predict the IRE-induced cell necrosis. Three-dimensionally (3-D) cultured cells in a tissue phantom were experimentally subjected to the electric pulses through a pair of puncture electrodes. The necrotized area was determined as a function of the pulse repetition and compared with the area that was estimated by the numerical analysis.

AB - Irreversible electroporation (IRE) is a new treatment to necrotize abnormal cells by high electric pulses. Electric potential difference over 1 V across the plasma membrane permanently permeabilizes the cell with keeping the extracellular matrix intact if the thermal damage due to the Joule heating effect is avoided. This is the largest advantage of the IRE compared to the other conventional treatment. However, since the IRE has just started to be used in clinical tests, it is important to predict the necrotized region that depends on pulse parameters and electrode arrangement. We therefore examined the numerical solution to the Laplace equation for the static electric field to predict the IRE-induced cell necrosis. Three-dimensionally (3-D) cultured cells in a tissue phantom were experimentally subjected to the electric pulses through a pair of puncture electrodes. The necrotized area was determined as a function of the pulse repetition and compared with the area that was estimated by the numerical analysis.

UR - http://www.scopus.com/inward/record.url?scp=84881061644&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881061644&partnerID=8YFLogxK

U2 - 10.1109/EMBC.2012.6345900

DO - 10.1109/EMBC.2012.6345900

M3 - Conference contribution

SN - 9781424441198

T3 - Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS

SP - 179

EP - 182

BT - 2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2012

ER -