TY - JOUR
T1 - Effect of light on carotenoid and lipid production in the oleaginous yeast Rhodosporidium toruloides
AU - Pham, Khanh Dung
AU - Shida, Yosuke
AU - Miyata, Atsushi
AU - Takamizawa, Takeru
AU - Suzuki, Yoshiyuki
AU - Ara, Satoshi
AU - Yamazaki, Harutake
AU - Masaki, Kazuo
AU - Mori, Kazuki
AU - Aburatani, Sachiyo
AU - Hirakawa, Hideki
AU - Tashiro, Kosuke
AU - Kuhara, Satoru
AU - Takaku, Hiroaki
AU - Ogasawara, Wataru
N1 - Funding Information:
A part of this work was supported by a grant from the New Energy and Industrial Technology Development Organization (NEDO) Project [P16009]. A part of this work was supported by the research fund of the Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry. We thank Dr. Yoshinori Takahara (Nagaoka University of Technology) for the experiment assistance.
Publisher Copyright:
© 2020, © 2020 Japan Society for Bioscience, Biotechnology, and Agrochemistry.
PY - 2020/7/2
Y1 - 2020/7/2
N2 - The oleaginous yeast Rhodosporodium toruloides is receiving widespread attention as an alternative energy source for biofuels due to its unicellular nature, high growth rate and because it can be fermented on a large-scale. In this study, R. toruloides was cultured under both light and dark conditions in order to understand the light response involved in lipid and carotenoid biosynthesis. Our results from phenotype and gene expression analysis showed that R. toruloides responded to light by producing darker pigmentation with an associated increase in carotenoid production. Whilst there was no observable difference in lipid production, slight changes in the fatty acid composition were recorded. Furthermore, a two-step response was found in three genes (GGPSI, CAR1, and CAR2) under light conditions and the expression of the gene encoding the photoreceptor CRY1 was similarly affected.
AB - The oleaginous yeast Rhodosporodium toruloides is receiving widespread attention as an alternative energy source for biofuels due to its unicellular nature, high growth rate and because it can be fermented on a large-scale. In this study, R. toruloides was cultured under both light and dark conditions in order to understand the light response involved in lipid and carotenoid biosynthesis. Our results from phenotype and gene expression analysis showed that R. toruloides responded to light by producing darker pigmentation with an associated increase in carotenoid production. Whilst there was no observable difference in lipid production, slight changes in the fatty acid composition were recorded. Furthermore, a two-step response was found in three genes (GGPSI, CAR1, and CAR2) under light conditions and the expression of the gene encoding the photoreceptor CRY1 was similarly affected.
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U2 - 10.1080/09168451.2020.1740581
DO - 10.1080/09168451.2020.1740581
M3 - Article
C2 - 32189572
AN - SCOPUS:85082318225
SN - 0916-8451
VL - 84
SP - 1501
EP - 1512
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 7
ER -