Effect of mechanical instability of polymer scaffolds on cell adhesion

Shinichiro Shimomura, Hisao Matsuno, Keiji Tanaka

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The adhesion of fibroblast on polymer bilayers composed of a glassy polystyrene (PS) prepared on top of a rubbery polyisoprene (PI) was studied. Since the top PS layer is not build on a glassy, or firm, foundation, the system becomes mechanically unstable with decreasing thickness of the PS layer. When the PS film was thinner than 25 nm, the number of cells adhered to the surface decreased and the cells could not spread well. On a parallel experiment, the same cell adhesion behavior was observed on plasma-treated PS/PI bilayer films, where in this case, the surface was more hydrophilic than that of the intact films. In addition, the fluorescence microscopic observations revealed that the formation of F-actin filaments in fibroblasts attached to the thicker PS/PI bilayer films was greater than those using the thinner PS/PI bilayer films. On the other hand, the thickness dependence of the cell adhesion behavior was not observed for the PS monolayer films. Taking into account that the amount of adsorbed protein molecules evaluated by a quartz crystal microbalance method was independent of the PS layer thickness of the bilayer films, our results indicate that cells, unlike protein molecules, could sense a mechanical instability of the scaffold.

Original languageEnglish
Pages (from-to)11087-11092
Number of pages6
JournalLangmuir
Volume29
Issue number35
DOIs
Publication statusPublished - Sep 10 2013

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Polystyrenes
Cell adhesion
Scaffolds (biology)
polystyrene
Polymers
adhesion
polyisoprenes
Polyisoprenes
polymers
cells
fibroblasts
Fibroblasts
proteins
Proteins
Molecules
Quartz crystal microbalances
quartz crystals
Scaffolds
microbalances
Actins

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Effect of mechanical instability of polymer scaffolds on cell adhesion. / Shimomura, Shinichiro; Matsuno, Hisao; Tanaka, Keiji.

In: Langmuir, Vol. 29, No. 35, 10.09.2013, p. 11087-11092.

Research output: Contribution to journalArticle

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