Effect of micro-roughening of poly(ether ether ketone) on bone marrow derived stem cell and macrophage responses, and osseointegration

Sunarso, Akira Tsuchiya, Naoyuki Fukuda, Riki Toita, Kanji Tsuru, Kunio Ishikawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Poly(ether ether ketone) (PEEK) has emerged as a candidate to replace metal implants because of its satisfactory mechanical properties, radiolucency, and lack of metal allergy. However, PEEK lacks osseointegration ability limiting its clinical applications. To overcome this problem, we prepared PEEK with a micro-rough surface using the sandblast method to modulate its osseointegration property; the sandblast method is simple, cost-effective, and is already applied to clinical metal implants. The surface roughness of the sandblasted PEEK was about 2.3 μm, whereas that of mirror-polished PEEK was 0.06 μm. Rat bone marrow-derived mesenchymal stem cells (RMSCs) showed higher proliferation, osteocalcin (OC) expression and bone-like nodule formation on micro-roughened PEEK compared with those cultured on mirror-polished PEEK, suggesting that micro-roughening facilitated RMSCs proliferation and differentiation. The micro-roughened surface slightly mitigated secretion of inflammatory C-C motif chemokine 2 (CCL-2) from lipopolysaccharide (LPS)-stimulated macrophages, but not of tumor necrosis factor α (TNFα) and interleukin-6 (IL-6). Finally, to compare osseointegration, specimens were implanted in rat femur bone marrow cavities, and then the pull-out force was measured. The pull-out force of micro-roughened PEEK was about four times higher than that of the mirror-polished PEEK. These results showed that micro-roughening of PEEK using the sandblast method was able to improve osseointegration, partly through elevating proliferation and differentiation of RMSCs.

Original languageEnglish
Pages (from-to)1375-1388
Number of pages14
JournalJournal of Biomaterials Science, Polymer Edition
Volume29
Issue number12
DOIs
Publication statusPublished - Aug 13 2018

Fingerprint

Osseointegration
Polyether ether ketones
Macrophages
Stem cells
Ketones
Ether
Bone
Stem Cells
Mirrors
Rats
Allergies
Metals
Osteocalcin
Chemokines
Lipopolysaccharides
Interleukin-6
Tumor Necrosis Factor-alpha
Bone Marrow
Surface roughness

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Effect of micro-roughening of poly(ether ether ketone) on bone marrow derived stem cell and macrophage responses, and osseointegration. / Sunarso, ; Tsuchiya, Akira; Fukuda, Naoyuki; Toita, Riki; Tsuru, Kanji; Ishikawa, Kunio.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 29, No. 12, 13.08.2018, p. 1375-1388.

Research output: Contribution to journalArticle

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