Effect of minerals on accumulation of Cs by fungus Saccaromyces cerevisiae

Toshihiko Ohnuki, Fuminori Sakamoto, Shinya Yamasaki, Naofumi Kozai, Hiroyuki Shiotsu, Satoshi Utsunomiya, Naoko Watanabe, Tamotsu Kozaki

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The accumulation of Cs by unicellular fungus of Saccharomyces cerevisiae in the presence of minerals has been studied to elucidate the role of microorganisms in the migration of radioactive Cs in the environment. Two different types of experiments were employed: experiments using stable Cs to examine the effect of a carbon source on the accumulation of Cs, and accumulation experiments of radioactive Cs from agar medium containing 137Cs and zeolite, vermiculite, phlogopite, smectite, mica, or illite as mineral supplements. In the former type of experiments, the Cs-accumulated cells were analyzed by scanning electron microscopy equipped with energy dispersive X-ray analysis (SEM-EDS). In the latter type, the radioactivity in the yeast cells was measured by an autoradiography technique. When a carbon source was present, higher amounts of Cs accumulated in the cells than in the resting condition without a carbon source. Analyses with SEM-EDS showed that no mineral formed on the cell surface. These results indicate that the yeast cells accumulate Cs by adsorption on the cell surface and intracellular accumulation. In the presence of minerals in the agar medium, the radioactivity in the yeast cells was in the order of mica>smectite, illite>>vermiculite, phlogopite, zeolite. This order is inversely correlated to the ratio of the concentration of radioactive Cs between the minerals and the medium solution. These results strongly suggest that the yeast accumulates radioactive Cs competitively with minerals.

Original languageEnglish
Pages (from-to)127-133
Number of pages7
JournalJournal of Environmental Radioactivity
Volume144
DOIs
Publication statusPublished - Jun 1 2015

Fingerprint

Fungi
Minerals
Yeast
fungus
yeast
mineral
Yeasts
Zeolites
Carbon
scanning electron microscopy
vermiculite
Cells
Mica
Radioactivity
phlogopite
radioactivity
agar
zeolite
mica
illite

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

Effect of minerals on accumulation of Cs by fungus Saccaromyces cerevisiae. / Ohnuki, Toshihiko; Sakamoto, Fuminori; Yamasaki, Shinya; Kozai, Naofumi; Shiotsu, Hiroyuki; Utsunomiya, Satoshi; Watanabe, Naoko; Kozaki, Tamotsu.

In: Journal of Environmental Radioactivity, Vol. 144, 01.06.2015, p. 127-133.

Research output: Contribution to journalArticle

Ohnuki, T, Sakamoto, F, Yamasaki, S, Kozai, N, Shiotsu, H, Utsunomiya, S, Watanabe, N & Kozaki, T 2015, 'Effect of minerals on accumulation of Cs by fungus Saccaromyces cerevisiae', Journal of Environmental Radioactivity, vol. 144, pp. 127-133. https://doi.org/10.1016/j.jenvrad.2015.02.018
Ohnuki, Toshihiko ; Sakamoto, Fuminori ; Yamasaki, Shinya ; Kozai, Naofumi ; Shiotsu, Hiroyuki ; Utsunomiya, Satoshi ; Watanabe, Naoko ; Kozaki, Tamotsu. / Effect of minerals on accumulation of Cs by fungus Saccaromyces cerevisiae. In: Journal of Environmental Radioactivity. 2015 ; Vol. 144. pp. 127-133.
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