The effect of the misfit angle formation on the frictional property of MoS2 film was studied. The accumulated frictional force increased as the misfit angle (θ) approaches θ = 0° or 60°, and decreased as it approached θ = 30°C. This result suggested that the misfit angle plays a critical role in the lubrication performance of the MoS2 film. The frictional force changed when the Coulombic energy was high, i.e., the upper sulfur layer approached to the lower layer. The frictional force was positive value when the direction of Coulombic force was opposite to the sliding direction. When the frictional force was negative, the Coulumbic force remained the same. These results indicated that the Coulombic energy between sulfur layer strongly affect the frictional force of MoS2 layer. This is an abstract of a paper presented at the World Tribology Congress (Kyoto, Japan 9/6-11/2009).