Effect of norepinephrine on rat basilar artery in vivo

T. Kitazono, F. M. Faraci, D. D. Heistad

Research output: Contribution to journalArticle

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Abstract

In anesthetized rats, we used a cranial window to examine effects of topical norepinephrine on diameter of the basilar artery in vivo. Topical application of norepinephrine increased the diameter of the basilar artery. N(G)-nitro-L-arginine methyl ester, an inhibitor of nitric oxide synthase, inhibited vasodilatation to acetylcholine but did not attenuate dilator responses to norepinephrine. Indomethacin also did not attenuate vasodilatation in response to norepinephrine. Dilatation of the basilar artery to norepinephrine was inhibited by propranolol and the β1-antagonist atenolol but not by the β2-antagonist butoxamine. Thus dilatation of the basilar artery in response to norepinephrine is produced by activation of β1-receptors and is not mediated by endothelium-derived relaxing factor or prostanoids. Glibenclamide, a selective inhibitor of ATP-sensitive K+ channels, partially inhibited vasodilatation in response to norepinephrine. Forskolin, a direct activator of adenylate cyclase, also increased the diameter of the basilar artery, and glibenclamide attenuated the dilatation. Thus dilatation of rat basilar artery in response to norepinephrine is mediated, in part, by activation of ATP-sensitive K+ channels, and activation of these K+ channels may be achieved by an adenosine 3',5'- cyclic monophosphate-dependent mechanism.

Original languageEnglish
Pages (from-to)H178-H182
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume264
Issue number1 33-1
Publication statusPublished - Feb 24 1993
Externally publishedYes

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Basilar Artery
Norepinephrine
Dilatation
Vasodilation
Glyburide
Butoxamine
Adenosine Triphosphate
Endothelium-Dependent Relaxing Factors
Atenolol
NG-Nitroarginine Methyl Ester
Colforsin
Adenylyl Cyclases
Propranolol
Nitric Oxide Synthase
Indomethacin
Cyclic AMP
Acetylcholine
Prostaglandins

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Effect of norepinephrine on rat basilar artery in vivo. / Kitazono, T.; Faraci, F. M.; Heistad, D. D.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 264, No. 1 33-1, 24.02.1993, p. H178-H182.

Research output: Contribution to journalArticle

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