Effect of nutrient intake on intramuscular glucose metabolism during the early growth stage in cross-bred steers (Japanese Black male×Holstein female)

F. Ebara, S. Inada, M. Morikawa, S. H. Asaoka, Y. Isozaki, A. Saito, T. Etoh, Y. Shiotsuka, S. G. Roh, J. Wegner, T. Gotoh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The objective was to investigate the impact of nutrient intake during the early growth period on the expression of glucose metabolism-related genes in skeletal muscle of cross-bred cattle. From 1.5 to 5months of age, group H (n=7) animals were intensively fed a high-protein and low-fat milk replacer [crude protein (CP) 28%; ether extracts (EE) 18%; max: 2.0kg, 12l/day], and group R (n=7) animals were fed a restricted amount of normal milk replacer (CP 25%; EE 23%; max 0.5kg, 4l/day). From 6 to 10months of age, group H cattle were fed a high-nutrition total mixed ration mainly prepared from grain feed, and group R cattle were fed only roughage. Blood samples were taken from each animal at three biopsy times (1.5, 5 and 10months of age), and the blood plasma concentration of glucose and insulin was analysed. In glucose concentration, there were no significant differences; however, the concentrations of insulin were higher in group H than in group R at 5 and 10months of age. Muscle samples were taken by biopsy from longissimus thoracis muscle (LT) at 1.5, 5 and 10months of age. We analysed mRNA expression levels using the quantitative real-time polymerase chain reaction (PCR) assay for glucose transporters (GLUT1 and GLUT4), insulin receptor, phosphatidylinositol 3-kinase (PI-3K), protein kinase B (PKB, also known as Akt), hexokinase 1 (HK1) and tumour necrosis factor alpha (TNFα). Although no differences were detected at 1.5 and 5months of age, at 10months of age, GLUT1, HK1 and TNFα mRNA expression levels were significantly higher in group H than in group R. These results suggested Glut1 that affects insulin-independently mediated glucose uptake was more responsive to improved nutrition during early growth stage than GLUT4 that insulin-dependently mediated glucose uptake in LT of cattle.

Original languageEnglish
Pages (from-to)684-693
Number of pages10
JournalJournal of Animal Physiology and Animal Nutrition
Volume97
Issue number4
DOIs
Publication statusPublished - Aug 1 2013

Fingerprint

Wagyu
nutrient intake
crossbreds
developmental stages
Glucose
Food
glucose
metabolism
Insulin
Growth
Hexokinase
insulin
Muscles
Ether
milk replacer
hexokinase
Milk
cattle
Age Groups
Tumor Necrosis Factor-alpha

All Science Journal Classification (ASJC) codes

  • Food Animals
  • Animal Science and Zoology

Cite this

Effect of nutrient intake on intramuscular glucose metabolism during the early growth stage in cross-bred steers (Japanese Black male×Holstein female). / Ebara, F.; Inada, S.; Morikawa, M.; Asaoka, S. H.; Isozaki, Y.; Saito, A.; Etoh, T.; Shiotsuka, Y.; Roh, S. G.; Wegner, J.; Gotoh, T.

In: Journal of Animal Physiology and Animal Nutrition, Vol. 97, No. 4, 01.08.2013, p. 684-693.

Research output: Contribution to journalArticle

Ebara, F. ; Inada, S. ; Morikawa, M. ; Asaoka, S. H. ; Isozaki, Y. ; Saito, A. ; Etoh, T. ; Shiotsuka, Y. ; Roh, S. G. ; Wegner, J. ; Gotoh, T. / Effect of nutrient intake on intramuscular glucose metabolism during the early growth stage in cross-bred steers (Japanese Black male×Holstein female). In: Journal of Animal Physiology and Animal Nutrition. 2013 ; Vol. 97, No. 4. pp. 684-693.
@article{e795bfd79f8342a89eb37e6c150a6850,
title = "Effect of nutrient intake on intramuscular glucose metabolism during the early growth stage in cross-bred steers (Japanese Black male×Holstein female)",
abstract = "The objective was to investigate the impact of nutrient intake during the early growth period on the expression of glucose metabolism-related genes in skeletal muscle of cross-bred cattle. From 1.5 to 5months of age, group H (n=7) animals were intensively fed a high-protein and low-fat milk replacer [crude protein (CP) 28{\%}; ether extracts (EE) 18{\%}; max: 2.0kg, 12l/day], and group R (n=7) animals were fed a restricted amount of normal milk replacer (CP 25{\%}; EE 23{\%}; max 0.5kg, 4l/day). From 6 to 10months of age, group H cattle were fed a high-nutrition total mixed ration mainly prepared from grain feed, and group R cattle were fed only roughage. Blood samples were taken from each animal at three biopsy times (1.5, 5 and 10months of age), and the blood plasma concentration of glucose and insulin was analysed. In glucose concentration, there were no significant differences; however, the concentrations of insulin were higher in group H than in group R at 5 and 10months of age. Muscle samples were taken by biopsy from longissimus thoracis muscle (LT) at 1.5, 5 and 10months of age. We analysed mRNA expression levels using the quantitative real-time polymerase chain reaction (PCR) assay for glucose transporters (GLUT1 and GLUT4), insulin receptor, phosphatidylinositol 3-kinase (PI-3K), protein kinase B (PKB, also known as Akt), hexokinase 1 (HK1) and tumour necrosis factor alpha (TNFα). Although no differences were detected at 1.5 and 5months of age, at 10months of age, GLUT1, HK1 and TNFα mRNA expression levels were significantly higher in group H than in group R. These results suggested Glut1 that affects insulin-independently mediated glucose uptake was more responsive to improved nutrition during early growth stage than GLUT4 that insulin-dependently mediated glucose uptake in LT of cattle.",
author = "F. Ebara and S. Inada and M. Morikawa and Asaoka, {S. H.} and Y. Isozaki and A. Saito and T. Etoh and Y. Shiotsuka and Roh, {S. G.} and J. Wegner and T. Gotoh",
year = "2013",
month = "8",
day = "1",
doi = "10.1111/j.1439-0396.2012.01310.x",
language = "English",
volume = "97",
pages = "684--693",
journal = "Journal of Animal Physiology and Animal Nutrition",
issn = "0931-2439",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Effect of nutrient intake on intramuscular glucose metabolism during the early growth stage in cross-bred steers (Japanese Black male×Holstein female)

AU - Ebara, F.

AU - Inada, S.

AU - Morikawa, M.

AU - Asaoka, S. H.

AU - Isozaki, Y.

AU - Saito, A.

AU - Etoh, T.

AU - Shiotsuka, Y.

AU - Roh, S. G.

AU - Wegner, J.

AU - Gotoh, T.

PY - 2013/8/1

Y1 - 2013/8/1

N2 - The objective was to investigate the impact of nutrient intake during the early growth period on the expression of glucose metabolism-related genes in skeletal muscle of cross-bred cattle. From 1.5 to 5months of age, group H (n=7) animals were intensively fed a high-protein and low-fat milk replacer [crude protein (CP) 28%; ether extracts (EE) 18%; max: 2.0kg, 12l/day], and group R (n=7) animals were fed a restricted amount of normal milk replacer (CP 25%; EE 23%; max 0.5kg, 4l/day). From 6 to 10months of age, group H cattle were fed a high-nutrition total mixed ration mainly prepared from grain feed, and group R cattle were fed only roughage. Blood samples were taken from each animal at three biopsy times (1.5, 5 and 10months of age), and the blood plasma concentration of glucose and insulin was analysed. In glucose concentration, there were no significant differences; however, the concentrations of insulin were higher in group H than in group R at 5 and 10months of age. Muscle samples were taken by biopsy from longissimus thoracis muscle (LT) at 1.5, 5 and 10months of age. We analysed mRNA expression levels using the quantitative real-time polymerase chain reaction (PCR) assay for glucose transporters (GLUT1 and GLUT4), insulin receptor, phosphatidylinositol 3-kinase (PI-3K), protein kinase B (PKB, also known as Akt), hexokinase 1 (HK1) and tumour necrosis factor alpha (TNFα). Although no differences were detected at 1.5 and 5months of age, at 10months of age, GLUT1, HK1 and TNFα mRNA expression levels were significantly higher in group H than in group R. These results suggested Glut1 that affects insulin-independently mediated glucose uptake was more responsive to improved nutrition during early growth stage than GLUT4 that insulin-dependently mediated glucose uptake in LT of cattle.

AB - The objective was to investigate the impact of nutrient intake during the early growth period on the expression of glucose metabolism-related genes in skeletal muscle of cross-bred cattle. From 1.5 to 5months of age, group H (n=7) animals were intensively fed a high-protein and low-fat milk replacer [crude protein (CP) 28%; ether extracts (EE) 18%; max: 2.0kg, 12l/day], and group R (n=7) animals were fed a restricted amount of normal milk replacer (CP 25%; EE 23%; max 0.5kg, 4l/day). From 6 to 10months of age, group H cattle were fed a high-nutrition total mixed ration mainly prepared from grain feed, and group R cattle were fed only roughage. Blood samples were taken from each animal at three biopsy times (1.5, 5 and 10months of age), and the blood plasma concentration of glucose and insulin was analysed. In glucose concentration, there were no significant differences; however, the concentrations of insulin were higher in group H than in group R at 5 and 10months of age. Muscle samples were taken by biopsy from longissimus thoracis muscle (LT) at 1.5, 5 and 10months of age. We analysed mRNA expression levels using the quantitative real-time polymerase chain reaction (PCR) assay for glucose transporters (GLUT1 and GLUT4), insulin receptor, phosphatidylinositol 3-kinase (PI-3K), protein kinase B (PKB, also known as Akt), hexokinase 1 (HK1) and tumour necrosis factor alpha (TNFα). Although no differences were detected at 1.5 and 5months of age, at 10months of age, GLUT1, HK1 and TNFα mRNA expression levels were significantly higher in group H than in group R. These results suggested Glut1 that affects insulin-independently mediated glucose uptake was more responsive to improved nutrition during early growth stage than GLUT4 that insulin-dependently mediated glucose uptake in LT of cattle.

UR - http://www.scopus.com/inward/record.url?scp=84880325644&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84880325644&partnerID=8YFLogxK

U2 - 10.1111/j.1439-0396.2012.01310.x

DO - 10.1111/j.1439-0396.2012.01310.x

M3 - Article

C2 - 22698257

AN - SCOPUS:84880325644

VL - 97

SP - 684

EP - 693

JO - Journal of Animal Physiology and Animal Nutrition

JF - Journal of Animal Physiology and Animal Nutrition

SN - 0931-2439

IS - 4

ER -