Effect of peroxisome proliferator-activated receptor-α ligands in the interaction between adipocytes and macrophages in obese adipose tissue

Takuya Toyoda, Yasutomi Kamei, Hirotsugu Kato, Satoshi Sugita, Motohiro Takeya, Takayoshi Suganami, Yoshihiro Ogawa

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Objective: This study was designed to examine the effect of peroxisome proliferator-activated receptor-α (PPAR-α) ligands on the inflammatory changes induced by the interaction between adipocytes and macrophages in obese adipose tissue. Methods and Procedures: PPAR-α ligands (Wy-14,643 and fenofibrate) were added to 3T3-L1 adipocytes, RAW264 macrophages, or co-culture of 3T3-L1 adipocytes and RAW264 macrophages in vitro, and monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-α (TNF-α) mRNA expression and secretion were examined. PPAR-α ligands were administered to genetically obese ob/ob mice for 2 weeks. Moreover, the effect of PPAR-α ligands was also evaluated in the adipose tissue explants and peritoneal macrophages obtained from PPAR-α-deficient mice. Results: In the co-culture of 3T3-L1 adipocytes and RAW264 macrophages, PPAR-α ligands reduced MCP-1 and TNF-α mRNA expression and secretion in vitro relative to vehicle-treated group. The anti-inflammatory effect of Wy-14,643 was observed in adipocytes treated with macrophage-conditioned media or mouse recombinant TNF-α and in macrophages treated with adipocyte-conditioned media or palmitate. Systemic administration of PPAR-α ligands inhibited the inflammatory changes in adipose tissue from ob/ob mice. Wy-14,643 also exerted an anti-inflammatory effect in the adipose tissue explants but not in peritoneal macrophages obtained from PPAR-α-deficient mice. Discussion: This study provides evidence for the anti-inflammatory effect of PPAR-α ligands in the interaction between adipocytes and macrophages in obese adipose tissue, thereby improving the dysregulation of adipocytokine production and obesity-related metabolic syndrome.

Original languageEnglish
Pages (from-to)1199-1207
Number of pages9
JournalObesity
Volume16
Issue number6
DOIs
Publication statusPublished - Jun 1 2008
Externally publishedYes

Fingerprint

Peroxisome Proliferator-Activated Receptors
Adipocytes
Adipose Tissue
Macrophages
Ligands
Anti-Inflammatory Agents
Tumor Necrosis Factor-alpha
Chemokine CCL2
Peritoneal Macrophages
Conditioned Culture Medium
Coculture Techniques
Fenofibrate
Messenger RNA
Adipokines
Palmitates
Obesity

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Nutrition and Dietetics

Cite this

Effect of peroxisome proliferator-activated receptor-α ligands in the interaction between adipocytes and macrophages in obese adipose tissue. / Toyoda, Takuya; Kamei, Yasutomi; Kato, Hirotsugu; Sugita, Satoshi; Takeya, Motohiro; Suganami, Takayoshi; Ogawa, Yoshihiro.

In: Obesity, Vol. 16, No. 6, 01.06.2008, p. 1199-1207.

Research output: Contribution to journalArticle

Toyoda, Takuya ; Kamei, Yasutomi ; Kato, Hirotsugu ; Sugita, Satoshi ; Takeya, Motohiro ; Suganami, Takayoshi ; Ogawa, Yoshihiro. / Effect of peroxisome proliferator-activated receptor-α ligands in the interaction between adipocytes and macrophages in obese adipose tissue. In: Obesity. 2008 ; Vol. 16, No. 6. pp. 1199-1207.
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