TY - JOUR
T1 - Effect of pore size of honeycomb films on the morphology, adhesion and cytoskeletal organization of cardiac myocytes
AU - Arai, Keiko
AU - Tanaka, Masaru
AU - Yamamoto, Sadaaki
AU - Shimomura, Masatsugu
N1 - Funding Information:
This work is supported by Grants-in-Aid from Japan Science and Technology Corporation (JST) and Special Coordination Funds for Promoting Science and Technology of Ministry of Education, Culture, Sports, Science and Technology.
PY - 2008/2/1
Y1 - 2008/2/1
N2 - Cells respond to the circumstances such as structures and chemical composition of scaffolds and cytokine. In this report, the responses of cytoskeleton and spreading of cardiac myocytes to the self-organized honeycomb-patterned films (honeycomb films) of biodegradable poly(ε-caprolactone) (PCL) with several pore sizes were investigated. The expression of actin filament of cardiac myocytes was strongly dependent on the pore size ranging from 4 to 13 μm. Immunofluorescent labeling of vinculin in cardiac myocytes showed localization of focal contact along the edge of the honeycomb pores. The results indicated that the honeycomb structures and the pore sizes influence the morphology, cytoskeletal organization and focal adhesion of the cardiac myocytes.
AB - Cells respond to the circumstances such as structures and chemical composition of scaffolds and cytokine. In this report, the responses of cytoskeleton and spreading of cardiac myocytes to the self-organized honeycomb-patterned films (honeycomb films) of biodegradable poly(ε-caprolactone) (PCL) with several pore sizes were investigated. The expression of actin filament of cardiac myocytes was strongly dependent on the pore size ranging from 4 to 13 μm. Immunofluorescent labeling of vinculin in cardiac myocytes showed localization of focal contact along the edge of the honeycomb pores. The results indicated that the honeycomb structures and the pore sizes influence the morphology, cytoskeletal organization and focal adhesion of the cardiac myocytes.
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U2 - 10.1016/j.colsurfa.2007.04.128
DO - 10.1016/j.colsurfa.2007.04.128
M3 - Article
AN - SCOPUS:37349064789
SN - 0927-7757
VL - 313-314
SP - 530
EP - 535
JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects
JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects
ER -