Effect of prolonged storage on the virulence of polyhedral inclusion body from seven nucleopolyhedrovirus isolates infectious to the tobacco cutworm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae)

Katsuo Tsuda, Kouhei Yamashita, Yositaka Sakamaki, Kanetosi Kusigemati, Chisa Yasunaga-Aoki, Kazuhiro Iiyama, Muneo Okada, Takeshi Kawarabata

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Polyhedral inclusion bodies (PIB's) of seven isolates of nucleopolyhedrovirus (NPV) (Spodoptera litura NPV Fukuyama, S. littoralis NPV Egypt, Mamestra brassicae NPV Geihoku, M. brassicae NPV Tokyo, Xestia c-nigrum NPV Nasu, Mythimna separata NPV Geihoku, and M. loreyi NPV Fukuyama) that have been known to infect the tobacco cutworm, S. litura, were stored as water suspensions at 4-6°C for more than 20 yr after preparation in cold storage. The virulence of PIBs was detected successfully from six NPV isolates, excluding M. loreyi NPV Fukuyama. PIBs of re-isolated viruses prepared from newly infected S. litura larvae were further used to estimate the initial infectivity of the stored PIBs. PIBs of S. litura NPV Fukuyama and S. littoralis NPV Egypt were relatively resistant to longterm storage and the infectivity decreased to 1/50 after 24-yr storage in distilled water. M. brassicae NPVs seemed highly sensitive to long-term storage under the conditions of the present study.

Original languageEnglish
Pages (from-to)119-122
Number of pages4
JournalJapanese Journal of Applied Entomology and Zoology
Volume49
Issue number3
DOIs
Publication statusPublished - Dec 12 2005

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Spodoptera litura
Baculoviridae
inclusion bodies
Noctuidae
virulence
Lepidoptera
Mamestra brassicae
Spodoptera litura nucleopolyhedrovirus
Egypt
Mythimna loreyi
Spodoptera littoralis nucleopolyhedrovirus
Xestia c-nigrum
pathogenicity
Mythimna separata
cold storage
water
viruses
larvae

All Science Journal Classification (ASJC) codes

  • Insect Science

Cite this

Effect of prolonged storage on the virulence of polyhedral inclusion body from seven nucleopolyhedrovirus isolates infectious to the tobacco cutworm, Spodoptera litura (Fabricius) (Lepidoptera : Noctuidae). / Tsuda, Katsuo; Yamashita, Kouhei; Sakamaki, Yositaka; Kusigemati, Kanetosi; Yasunaga-Aoki, Chisa; Iiyama, Kazuhiro; Okada, Muneo; Kawarabata, Takeshi.

In: Japanese Journal of Applied Entomology and Zoology, Vol. 49, No. 3, 12.12.2005, p. 119-122.

Research output: Contribution to journalArticle

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