Effect of Rose Bengal on Immunoglobulin Production by Mouse B Lymphoma, WEHI-279 Cells

Yuichiro Kuramoto, Yoshiyuki Miyazaki, Shinichiro Inoue, Hirotaka Haruta, Hirofumi Tachibana, Michihiro Sugano, Koji Yamada

Research output: Contribution to journalArticle

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Abstract

The effect of Rose Bengal (RB) on immunoglobulin (Ig) production by mouse B lymphoma WEHI-279 cells was examined. At 100 μM, RB increased IgE concentration in the culture medium but decreased the levels of IgG and IgM. To examine whether RB affects to the secretion of IgE from WEHI-279 cells or its synthesis, intracellular, and extracellular Ig levels were compared. The cytoplasmic IgE level in the cells treated with 100 μM RB was higher than in the cells cultured without RB. Reverse transcriptase-polymerase chain reaction (RT-PCR) experiment showed that the level of productive ∈ transcripts of the RB-added group was higher than that of the control but RB did not affect the germ-line ∈ transcripts. These results suggested that RB enhancement of IgE was due to the increase of the Ig synthesis by WEHI-279 cells and not to the increase of secretion activity of Ig from the cells nor to the enhancement of class-switching to IgE producing cells.

Original languageEnglish
Pages (from-to)5368-5372
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume46
Issue number12
DOIs
Publication statusPublished - Jan 1 1998

Fingerprint

Rose Bengal
lymphoma
immunoglobulins
Immunoglobulins
Lymphoma
Immunoglobulin E
mice
cells
secretion
Immunoglobulin Class Switching
synthesis
rose bengal
Polymerase chain reaction
RNA-Directed DNA Polymerase
Reverse Transcriptase Polymerase Chain Reaction
Germ Cells
cultured cells
Immunoglobulin M
Culture Media
Cultured Cells

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Effect of Rose Bengal on Immunoglobulin Production by Mouse B Lymphoma, WEHI-279 Cells. / Kuramoto, Yuichiro; Miyazaki, Yoshiyuki; Inoue, Shinichiro; Haruta, Hirotaka; Tachibana, Hirofumi; Sugano, Michihiro; Yamada, Koji.

In: Journal of Agricultural and Food Chemistry, Vol. 46, No. 12, 01.01.1998, p. 5368-5372.

Research output: Contribution to journalArticle

Kuramoto, Yuichiro ; Miyazaki, Yoshiyuki ; Inoue, Shinichiro ; Haruta, Hirotaka ; Tachibana, Hirofumi ; Sugano, Michihiro ; Yamada, Koji. / Effect of Rose Bengal on Immunoglobulin Production by Mouse B Lymphoma, WEHI-279 Cells. In: Journal of Agricultural and Food Chemistry. 1998 ; Vol. 46, No. 12. pp. 5368-5372.
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