Effect of Rose Bengal on Immunoglobulin Production by Mouse B Lymphoma, WEHI-279 Cells

Yuichiro Kuramoto, Yoshiyuki Miyazaki, Shinichiro Inoue, Hirotaka Haruta, Hirofumi Tachibana, Michihiro Sugano, Koji Yamada

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Abstract

The effect of Rose Bengal (RB) on immunoglobulin (Ig) production by mouse B lymphoma WEHI-279 cells was examined. At 100 μM, RB increased IgE concentration in the culture medium but decreased the levels of IgG and IgM. To examine whether RB affects to the secretion of IgE from WEHI-279 cells or its synthesis, intracellular, and extracellular Ig levels were compared. The cytoplasmic IgE level in the cells treated with 100 μM RB was higher than in the cells cultured without RB. Reverse transcriptase-polymerase chain reaction (RT-PCR) experiment showed that the level of productive ∈ transcripts of the RB-added group was higher than that of the control but RB did not affect the germ-line ∈ transcripts. These results suggested that RB enhancement of IgE was due to the increase of the Ig synthesis by WEHI-279 cells and not to the increase of secretion activity of Ig from the cells nor to the enhancement of class-switching to IgE producing cells.

Original languageEnglish
Pages (from-to)5368-5372
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume46
Issue number12
DOIs
Publication statusPublished - Dec 1998

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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