Effect of salt and heating on a mesoscopic structure composed of ovalbumin globules in aqueous solution

M. Sugiyama, A. Nakamura, N. Hiramatsu, M. Annaka, S. Kuwajima, K. Hara

Research output: Contribution to journalArticle

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Abstract

Mesoscopic structural changes of an ovalbumin solution by heating and adding NaCl have been investigated with a small-angle neutron scattering method. In the natural solution, a broad peak at q = 0.057 Å-1, which disappeared by adding NaCl, indicates the existence of an electrostatic long-range interaction between the ovalbumin globules. Along with the broad peak, a prominent intensity increase in a very small q region was observed on heating except for the initial and final stages, indicating the coexistence of a disordered structure of the denatured ovalbumin and a regular-interspacing structure of the natural ovalbumin globules. Though the macroscopic feature in the final stage, whether the solution forms a gel (10 wt %) or not (5 wt %), strongly depended on the concentration of the ovalbumin, the scattering profiles showed a common characteristic feature: the appearance of a new peak around q = 0.023 Å-1, which indicates the emergence of another regular structure.

Original languageEnglish
Pages (from-to)1071-1073
Number of pages3
JournalBiomacromolecules
Volume2
Issue number4
DOIs
Publication statusPublished - Dec 1 2001

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Ovalbumin
Heating
Salts
Neutron scattering
Electrostatics
Small Angle Scattering
Gels
Scattering
Neutrons
Static Electricity

All Science Journal Classification (ASJC) codes

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Effect of salt and heating on a mesoscopic structure composed of ovalbumin globules in aqueous solution. / Sugiyama, M.; Nakamura, A.; Hiramatsu, N.; Annaka, M.; Kuwajima, S.; Hara, K.

In: Biomacromolecules, Vol. 2, No. 4, 01.12.2001, p. 1071-1073.

Research output: Contribution to journalArticle

Sugiyama, M. ; Nakamura, A. ; Hiramatsu, N. ; Annaka, M. ; Kuwajima, S. ; Hara, K. / Effect of salt and heating on a mesoscopic structure composed of ovalbumin globules in aqueous solution. In: Biomacromolecules. 2001 ; Vol. 2, No. 4. pp. 1071-1073.
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AU - Kuwajima, S.

AU - Hara, K.

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