Effect of SCCA1 and SCCA2 on the suppression of TNF-α-induced cell death by impeding the release of mitochondrial cytochrome c in an oral squamous cell carcinoma cell line

Ken Ichiro Hashimoto, Tamotsu Kiyoshima, Kou Matsuo, Satoru Ozeki, Hidetaka Sakai

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

This study examined the effect of squamous cell carcinoma antigen 1 (SCCA1) and SCCA2 on TNF-α-induced cell death in human oral squamous cell carcinoma cell lines. The viability of MISK81-5 and sMISK cells treated with TNF-α dose-dependently decreased. The sMISK cells which stably overexpressed SCCA1 and SCCA2 cDNA showed a greater resistance against the cell death induced by TNF-α than the controls. Immunocytochemical staining for cytochrome c showed a punctate pattern in the cytoplasm of the TNF-α-untreated cells. After treatment with TNF-α, the punctate staining pattern was preserved in the transfectants, while this pattern disappeared in the controls. In the transfectants, the release of cytochrome c from the mitochondria to cytosol after TNF-α treatment was lower than in the controls. A decreased level of active caspase-9 was also observed in the transfectants. These results suggest that both SCCA1 and SCCA2 play a role in the prevention of TNF-α-induced cell death in vitro, by inhibiting the release of mitochondrial cytochrome c to some degree.

Original languageEnglish
Pages (from-to)165-172
Number of pages8
JournalTumor Biology
Volume26
Issue number4
DOIs
Publication statusPublished - Jul 2005

All Science Journal Classification (ASJC) codes

  • Cancer Research

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