Effect of shear stress on microvessel network formation of endothelial cells with in vitro three-dimensional model

Akinori Ueda, Masaki Koga, Mariko Ikeda, Susumu Kudo, Kazuo Tanishita

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Shear stress stimulus is expected to enhance angiogenesis, the formation of microvessels. We determined the effect of shear stress stimulus on three-dimensional microvessel formation in vitro. Bovine pulmonary microvascular endothelial cells were seeded onto collagen gels with basic fibroblast growth factor to make a microvessel formation model. We observed this model in detail using phase-contrast microscopy, confocal laser scanning microscopy, and electron microscopy. The results show that cells invaded the collagen gel and reconstructed the tubular structures, containing a clearly defined lumen consisting of multiple cells. The model was placed in a parallel-plate flow chamber. A laminar shear stress of 0.3 Pa was applied to the surfaces of the cells for 48 h. Promotion of microvessel network formation was detectable after ∼10 h in the flow chamber. After 48 h, the length of networks exposed to shear stress was 6.17 (±0.59) times longer than at the initial state, whereas the length of networks not exposed to shear stress was only 3.30 (±0.41) times longer. The number of bifurcations and endpoints increased for networks exposed to shear stress, whereas the number of bifurcations alone increased for networks not exposed to shear stress. These results demonstrate that shear stress applied to the surfaces of endothelial cells on collagen gel promotes the growth of microvessel network formation in the gel and expands the network because of repeated bifurcation and elongation.

Original languageEnglish
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume287
Issue number3 56-3
DOIs
Publication statusPublished - Sep 1 2004
Externally publishedYes

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Microvessels
Endothelial Cells
Gels
Collagen
Phase-Contrast Microscopy
Fibroblast Growth Factor 2
Confocal Microscopy
Electron Microscopy
In Vitro Techniques
Lung
Growth

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

Effect of shear stress on microvessel network formation of endothelial cells with in vitro three-dimensional model. / Ueda, Akinori; Koga, Masaki; Ikeda, Mariko; Kudo, Susumu; Tanishita, Kazuo.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 287, No. 3 56-3, 01.09.2004.

Research output: Contribution to journalArticle

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