Effect of space flight on the frequency of micronuclei and expression of stress-responsive proteins in cultured mammalian cells.

Mituo Ikenaga, Jun Hirayama, Tomohisa Kato, Hiroyuki Kitao, Zhen Bo Han, Kanji Ishizaki, Kimiko Nishizawa, Fumio Suzuki, Thomas F. Cannon, Keiji Fukui, Toru Shimazu, Shigeki Kamigaichi, Noriaki Ishioka, Hiroyuki Matsumiya

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Results of past space experiments suggest that the biological effect of space radiation could be enhanced under microgravity in some cases, especially in insects. To examine if such a synergistic effect of radiation and microgravity also exists in human cells, frequencies of chromosome instability and cellular levels of several stress-responsive proteins were analyzed in cultured human and rodent cells after space flight. Human (MCF7 and AT2KY), mouse (m5S) and hamster (SHE) cell lines were loaded on the Space Shuttle Discovery (STS-95 mission) and grown during a 9-day mission. After landing, the micronuclei resulting from abnormal nuclear division and accumulation of stress-responsive proteins such as p53 and mitogen-activated protein kinases (MAPKs), which are involved in radiation-induced signal transduction cascades, were analyzed. The frequencies of micronuclei in all the four mammalian cell strains tested were not significantly different between flight and ground control samples. Also, the cellular amounts of p53, p21 (WAF1/SDI1/CIP1) and activated (phosphorylated) forms of three distinct MAPKs in MCF7 and m5S cells of flight samples were similar to those of ground control samples. These results indicated that any effect of space radiation, microgravity, or combination of both were not detectable, at least under the present experimental conditions.

Original languageEnglish
JournalJournal of radiation research
Volume43 Suppl
Publication statusPublished - Jan 1 2002
Externally publishedYes

Fingerprint

Weightlessness
Space Flight
space flight
Radiation Effects
Heat-Shock Proteins
cultured cells
Cultured Cells
microgravity
Mitogen-Activated Protein Kinases
proteins
extraterrestrial radiation
Cell Nucleus Division
Chromosomal Instability
MCF-7 Cells
Cricetinae
flight control
rodents
space transportation system
space shuttles
biological effects

All Science Journal Classification (ASJC) codes

  • Radiation
  • Radiology Nuclear Medicine and imaging
  • Health, Toxicology and Mutagenesis

Cite this

Effect of space flight on the frequency of micronuclei and expression of stress-responsive proteins in cultured mammalian cells. / Ikenaga, Mituo; Hirayama, Jun; Kato, Tomohisa; Kitao, Hiroyuki; Han, Zhen Bo; Ishizaki, Kanji; Nishizawa, Kimiko; Suzuki, Fumio; Cannon, Thomas F.; Fukui, Keiji; Shimazu, Toru; Kamigaichi, Shigeki; Ishioka, Noriaki; Matsumiya, Hiroyuki.

In: Journal of radiation research, Vol. 43 Suppl, 01.01.2002.

Research output: Contribution to journalArticle

Ikenaga, M, Hirayama, J, Kato, T, Kitao, H, Han, ZB, Ishizaki, K, Nishizawa, K, Suzuki, F, Cannon, TF, Fukui, K, Shimazu, T, Kamigaichi, S, Ishioka, N & Matsumiya, H 2002, 'Effect of space flight on the frequency of micronuclei and expression of stress-responsive proteins in cultured mammalian cells.', Journal of radiation research, vol. 43 Suppl.
Ikenaga, Mituo ; Hirayama, Jun ; Kato, Tomohisa ; Kitao, Hiroyuki ; Han, Zhen Bo ; Ishizaki, Kanji ; Nishizawa, Kimiko ; Suzuki, Fumio ; Cannon, Thomas F. ; Fukui, Keiji ; Shimazu, Toru ; Kamigaichi, Shigeki ; Ishioka, Noriaki ; Matsumiya, Hiroyuki. / Effect of space flight on the frequency of micronuclei and expression of stress-responsive proteins in cultured mammalian cells. In: Journal of radiation research. 2002 ; Vol. 43 Suppl.
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