Effect of Surface Microphase-Separated Structure on Interaction Between Biological Components and Multiphase Polymer Surface

Atsushi Takahara, Kinzo Korehisa, Shou Ren Ge, Tisato Kajiyama

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Polystyrene-poly(butadiene-co-hydroxylated butadiene)-polystyrene triblock copolymer (SHBS) with hydrophobic-hydrophilic microdomain structure has been prepared through the hydroxylation of polybutadiene (PBD) block of anionically polymerized SBS triblock copolymer. X-ray photoelectron spectroscopic and contact angle measurements revealed that the environmentally induced surface reorganization took place after exposure of the film to water in the case of low degree of hydroxylation of PBD block. The interaction between plasma protein and the SHBS surface has been studied on the basis of transmission electron microscopic observations of the specimen after immersing it in human serum albumin (HSA) and human fibrinogen (HFN) solutions. The adsorbed HSA and HFN were labeled with colloidal gold and the modified PBD block was stained with osmium tetroxide. Thus, the domain recognition of plasma protein can be analyzed. The amount of plasma protein adsorbed per unit area on PS domain did not depend on the degree of hydroxylation of PBD block. However, the amount of plasma protein adsorbed on the hydroxylated PBD block decreased with an increase in degree of hydroxylation. These behaviors can be ascribed to the selective protein adsorption onto hydrophobic phase in order to minimize the interfacial-free energy between polymer surface and plasma protein solution.

Original languageEnglish
Pages (from-to)2956-2961
Number of pages6
JournalJournal of Vacuum Science and Technology A: Vacuum, Surfaces and Films
Volume12
Issue number5
DOIs
Publication statusPublished - Sep 1994

All Science Journal Classification (ASJC) codes

  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films

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