Effect of temperature on the inhibition of the GABA-gated response by intracellular calcium

Tooru Maruyama, Yoshimi Ikemoto, Norio Akaike

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Abstract

The effects of the voltage-dependent Ca2+inward current (ICa) on γ-aminobutyric acid (GABA)-induced Cl- current (ICl) in isolated sensory neurons were examined at different temperatures and with different concentrations of external Ca2+ using the 'concentration-clamp' technique. The total amount of inhibition of GABA-induced ICl by a preceding ICa increased in a hyperbolic manner with increasing Ca2+ influx. The time course of recovery of the GABA response after inhibition by Ca 2+influx followed a single exponential and was facilitated by warming but slowed dramatically by a slight cooling from 20 to 15 °C in spite of a decrease in Ca2+ influx. It is discussed that the energy-dependent, temperature-sensitive ionic pump and exchange systems at the surface membrane and intracellular organelles regulate the cytoplasmic free Ca2+, thus explaining the quantitative effects of Ca2+ influx and temperature on the inhibition of the GABA-gated Cl- channel.

Original languageEnglish
Pages (from-to)17-22
Number of pages6
JournalBrain Research
Volume507
Issue number1
DOIs
Publication statusPublished - Jan 15 1990
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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